C0543

Rat Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

• Szinonimák: Type IV collagen, EHS-collagen
• CAS-szám: 9007-34-5
• EC-szám: 232-697-4
• MDL-szám: MFCD00130825
• UNSPSC kód: 12352202
• NACRES: NA.75

Tulajdonságok

• Terméknév: Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
• biológiai forrás: mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
• Minőségi szint: 200
• típus: Type IV (Miller)
• termékcsalád: BioReagent
• Forma: lyophilized powder
• kiszerelés: glass bottle of 1 vial
• technika/technikák: cell culture | mammalian: suitable
• felület bevonatolás: 6‑10 μg/cm²
• oldhatóság: 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
• UniProt elérési szám: B1AVK5; P02463; P08122; Q63ZW6; Q9QZS0
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• Géninformáció: mouse ... Col4a1(12826) , Col4a2(12827) , Col4a3(12828) , Col4a5(12830) , Col4a6(94216)

Leírás

Általános leírás

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Alkalmazás

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:

• in adhesion assays.
• in hydrogel fabrication.
• to coat cell culture plates for cell differentiation.
• to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.

Recommended for use as a cell culture substratum at 6-10 μg/cm².

Biokémiai/fiziológiai hatások

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Egyéb megjegyzések

Native collagen

We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Mennyiség

0.75 mg/vial

Elkészítési megjegyzés

Chloroform treated.
Pepsin is not used in the preparation.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)