C7521

Human Collagen Type IV

from human placenta, powder, suitable for substrate for collagenase and for diagnostic assay manufacturing

• Szinonimák: Collagen Type 4
• CAS-szám: 9007-34-5
• EC-szám: 232-697-4
• MDL-szám: MFCD00130825
• UNSPSC kód: 12352202
• NACRES: NA.54

Tulajdonságok

• Terméknév: Collagen from human placenta, Bornstein and Traub Type IV, powder
• biológiai forrás: human placenta
• Minőségi szint: 200
• leírás: Sigma Type VI
• Forma: powder
• szennyeződések: HIV, hepatitis B and hepatitis C, none detected
• oldhatóság: 0.5 M acetic acid: 1 mg/mL
• alkalmasság: suitable for substrate for collagenase
• UniProt elérési szám: P08572; P29400; P53420; Q01955; Q14031
• alkalmazás(ok): diagnostic assay manufacturing
• tárolási hőmérséklet: −20°C
• Géninformáció: human ... COL4A2(1284) , COL4A3(1285) , COL4A4(1286) , COL4A5(1287) , COL4A6(1288)

Leírás

Biokémiai/fiziológiai hatások

During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.

Komponensek

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.

Elkészítési megjegyzés

This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It is prepared by a modification of the pepsin extraction method of Glanville, R.W., et al.

Analízis megjegyzés

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Egyéb megjegyzések

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)