Összes fotó(1)
Fontos dokumentumok
11179179001
Roche
Collagen
from rat tail tendon
Szinonimák:
collagen
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352207
Javasolt termékek
biológiai forrás
rat tail
Minőségi szint
sterilitás
sterile
Forma
lyophilized (clear, colorless solution after reconstitution)
kiszerelés
pkg of 30 mg
gyártó/kereskedő neve
Roche
technika/technikák
cell culture | mammalian: suitable
UniProt elérési szám
tárolási hőmérséklet
2-8°C
Géninformáció
rat ... Col1a1(29393)
Általános leírás
Collagens are abundantly present in vertebrates. 41 specific genes codes for 42 polypeptide chains and produce 27 collagen types. Collagens are mainly present in the extracellular matrix. Collagen type I is abundantly present in mammals. Type IV collagen is an important structural protein of basement membranes. It is chemically and genetically different from stroma collagen types I and III and cartilage collagen type II.
Egyediség
Active on most mammalian cells.
Alkalmazás
Collagen has been used in the preparation of coverslip coating solution and collagen gel contraction assay.
Collagen is used as a substrate for culturing a variety of cells, such as coating of culture dishes and the preparation of collagen gels.
Biokémiai/fiziológiai hatások
Collagen provides extracellular support for multicellular animals. Collagen type I offers mechanical stability, strength and toughness to a range of tissues from tendons and ligaments, to skin, cornea, bone and dentin.
Tulajdonságok és előnyök
Contents
Lyophilizate, cell culture grade, 3 vials of 10mg
Lyophilizate, cell culture grade, 3 vials of 10mg
Specifikációk
Biological activity: Tested for the promotion of adherence of HUV-EC cells.
Elkészítési megjegyzés
Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.
5 μg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.
Feloldás
IFor best results, dissolve the lyophilizate in 0.2% acetic acid (v/v) solution.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.
Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.
Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
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