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Merck

A7888

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, suitable for RIA, pH 5.2, ≥96%

Szinonimák:

Albumin bovine serum, BSA, Bovine albumin

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

CAS-szám:
EC-szám:
MDL-szám:
UNSPSC kód:
12352202
NACRES:
NA.27

biológiai forrás

bovine

Minőségi szint

grade

RIA grade

Teszt

≥96%

form

lyophilized powder

minőség

suitable for RIA

molekulatömeg

~66 kDa

tisztítva

heat shock fractionation

kiszerelés

poly bottle of

eredet

USA origin

technika/technikák

blood typing: suitable

veszteség

≤5%

pH

5.2

oldhatóság

water: soluble (40 mg/ml)

alkalmasság

suitable for insulin RIA procedures

UniProt elérési szám

idegen aktivitás

BT virus, none detected
VSV virus, none detected

tárolási hőmérséklet

2-8°C

Géninformáció

bovine ... ALB(280717)

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Általános leírás

Bovine serum albumin (BSA) is 66 kDa protein consisting of three domains with two subdomains under each. It is α-helical globular and a non-glycosylated protein.

Alkalmazás

Bovine Serum Albumin has been used:
  • as a blocking agent in enzyme linked immunosorbent assay (ELISA)(154)
  • as a component of Dulbecco PBS medium for preantral follicles culture(155)
  • as a component of modified Tris-buffered medium (mTBM) for oocyte insemination(156)

Biokémiai/fiziológiai hatások

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. The cysteine and tyrosine residues of BSA have high affinity for metal binding.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Tulajdonságok és előnyök

  • Heat shock fractionated
  • Suitable for radioimmunoassay (RIA)

Vigyázat

May yield an insulin blank of up to 0.1 μunit per mg in certain procedures.

Elkészítési megjegyzés

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Yankel Chekli et al.
Scientific reports, 10(1), 15791-15791 (2020-09-27)
Bacterial proteins exported to the cell surface play key cellular functions. However, despite the interest to study the localisation of surface proteins such as adhesins, transporters or hydrolases, monitoring their dynamics in live imaging remains challenging, due to the limited
Lara Vlietstra et al.
PloS one, 14(6), e0217462-e0217462 (2019-06-07)
To determine if there is an association between sarcopenia, physical function and self-reported fatigue in osteoarthritis (OA) and rheumatoid arthritis (RA). A cross-sectional analysis of measurements from a cohort of 157 participants with OA or RA was performed. The relationship
K B Cullberg et al.
Nutrition & diabetes, 4, e113-e113 (2014-03-26)
High levels of free fatty acids (FFA) have been suggested to be one of the underlying mechanisms for adipose tissue (AT) inflammation and dysfunction in obesity. Human AT produces several adipokines including monocyte chemoattractant protein-1 (MCP-1), which are involved in
Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)?not all BSAs are alike
Xiao Y and Isaacs SN
Journal of Immunological Methods, 384(1-2), 148-151 (2012)
Sampada Chande et al.
Scientific reports, 10(1), 3069-3069 (2020-02-23)
Low blood phosphate (Pi) reduces muscle function in hypophosphatemic disorders. Which Pi transporters are required and whether hormonal changes due to hypophosphatemia contribute to muscle function is unknown. To address these questions we generated a series of conditional knockout mice

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