A3912

Bovine Serum Albumin

heat shock fraction, pH 5.2, ≥96%

• Szinonimák: Albumin bovine serum, BSA, Bovine albumin
• CAS-szám: 9048-46-8
• EC-szám: 232-936-2
• MDL-szám: MFCD00130384
• UNSPSC kód: 12352202
• NACRES: NA.27

Tulajdonságok

• biológiai forrás: bovine
• Minőségi szint: 200
• Teszt: ≥96%
• Forma: lyophilized powder
• molekulatömeg: ~66 kDa
• tisztítva: heat shock fractionation
• kiszerelés: poly bottle of
• eredet: USA origin
• technika/technikák: blood typing: suitable; microbiological culture: suitable
• veszteség: ≤5%
• pH: 5.2
• oldhatóság: water: soluble (40 mg/ml)
• UniProt elérési szám: P02769
• idegen aktivitás: BT Virus, none detected; VSV Virus, none detected
• tárolási hőmérséklet: 2-8°C
• Géninformáció: bovine ... ALB(280717)

Leírás

Általános leírás

Bovine serum albumin (BSA) is a 66.4 kDa, water-soluble protein composed of 583 amino acids. It has a single polypeptide chain with three homologous domains formed by six α-helices. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. BSA has been used as a model protein in various applications, including immunodiagnostic procedures, cell culture media and clinical chemistry. Serum albumins facilitates drug disposition and efficacy by maintaining osmotic blood pressure.

Alkalmazás

Bovine Serum Albumin (BSA) has been used:

• to study its adsorption on a planar poly(acrylic acid) (PAA) brush layer through fixed-angle optical reflectometry
• to measure its adsorption on a variety of thin films by means of total internal reflection spectroscopy
• as a constituent of Krebs-Ringer bicarbonate buffer, which acts as a perfusate during placental perfusion

Biokémiai/fiziológiai hatások

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Minőség

Remainder mostly globulins.

Elkészítési megjegyzés

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)