Összes fotó(1)
Fontos dokumentumok
A0731
Anti-ATG5 (C-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody
Szinonimák:
Anti-Atg5 (C-terminal) antibody produced in rabbit, Anti-ASP, Anti-Apg5, Anti-Apg5L, Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
konjugátum:
unconjugated
application:
ICC
WB
WB
klón:
polyclonal
faj reaktivitás:
mouse, human
citations:
67
technika/technikák:
immunocytochemistry: suitable
western blot: 1-2 μg/mL using whole extract of mouse liver or human U87 cells
western blot: 1-2 μg/mL using whole extract of mouse liver or human U87 cells
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
konjugátum
unconjugated
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
molekulatömeg
antigen ~56 kDa
faj reaktivitás
mouse, human
kiszerelés
antibody small pack of 25 μL
koncentráció
~1 mg/mL
technika/technikák
immunocytochemistry: suitable
western blot: 1-2 μg/mL using whole extract of mouse liver or human U87 cells
UniProt elérési szám
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... ATG5(9474)
mouse ... Atg5(11793)
Általános leírás
ATG5 (autophagy related 5) is located on human chromosome band 6q21.
ATG5 was found localized to double FYVE domain containing protein 1 (DFCP1)- specific omegasomes.
Macroautophagy, usually referred to as autophagy, is a major pathway for bulk degradation of cytoplasmic constituents and organelles. Autophagy is an evolutionary conserved pathway seen in all eukaryotic cells. At least 16 ATG genes, required for autophagosome formation were identified in yeast by genetic screens. For many of these genes, related homologs have been identified in mammals. Atg5, also known as Apg5, is a 32 kDa protein essential for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. This conjugation reaction requires ATP and two enzymes, Atg7 and Atg10, which are E1- and E2-like enzymes, respectively. The Atg12-Atg5 conjugate interacts non-covalently with Atg16. The Atg12-Atg5-Atg16 complex localizes to autophagosome precursors and plays an essential role in autophagosome formation. It has been reported that in addition to the role of Atg5 in the formation of autophagosomes, an Atg5 fragment produced by calpain cleavage has pro-apoptotic properties.
Rabbit polyclonal anti-ATG5 (C-terminal) antibody recognizes mouse and human Atg5-Atg12 complex by immunoblotting, ~56 kDa. Detection of the Atg5-Atg12 complex band by immunoblotting is specifically inhibited with the immunizing peptide.
Immunogén
synthetic peptide corresponding to amino acids 262-275 of mouse Atg5, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by one amino acid in human.
Alkalmazás
Anti-ATG5 (C-terminal) antibody has been used in immunohistochemical (IHC) staining.
Rabbit anti-ATG5 (C-terminal) antibody has been used for western blot analysis in U2OS cells. The antibody has also been used for immunoblot assays in CT26 and MCA205 cells.
Rabbit polyclonal anti-ATG5 (C-terminal) antibody is used to tag Atg5-Atg12 complex for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Atg5-Atg12 complex in the formation and function of autophagosomes. Rabbit anti-ATG5 was used as primary antibody for immunocytochemistry on HEK293 and Cos7 cells. Cells were fixed in 3.7% formaldehyde in 200 mM Hepes prior to antibody treatment.
Fizikai forma
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
kapcsolódó termék
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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Az ügyfelek ezeket is megtekintették
Elizabeth L Axe et al.
The Journal of cell biology, 182(4), 685-701 (2008-08-30)
Autophagy is the engulfment of cytosol and organelles by double-membrane vesicles termed autophagosomes. Autophagosome formation is known to require phosphatidylinositol 3-phosphate (PI(3)P) and occurs near the endoplasmic reticulum (ER), but the exact mechanisms are unknown. We show that double FYVE
Ashkan Mortezavi et al.
Oncotarget, 8(19), 31765-31774 (2017-04-21)
Autophagy is a catabolic cellular process used for degradation of cytoplasmic organelles and preservation of cell viability. In this study we aimed to analyse the level of autophagy markers in benign and malignant prostate tissue and to evaluate the prognostic
S Polager et al.
Oncogene, 27(35), 4860-4864 (2008-04-15)
The retinoblastoma pathway is often inactivated in human tumors resulting in deregulated E2F activity that can induce both proliferation and cell death. Although the role of E2F in apoptosis is well characterized, little is known regarding its putative participation in
Metastatic risk and resistance to BRAF inhibitors in melanoma defined by selective allelic loss of ATG5
Garcia F M, et al.
Autophagy, 12(10), 1776-1790 (2016)
Ying Gao et al.
Cell death & disease, 7(8), e2330-e2330 (2016-08-12)
Autophagy is an evolutionarily conserved catabolic process by which cells degrade intracellular proteins and organelles in the lysosomes. Canonical autophagy requires all autophagy proteins (ATGs), whereas noncanonical autophagy is activated by diverse agents in which some of the essential autophagy
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