10104159001
Roche
DNase I
grade II, from bovine pancreas
Szinonimák:
Deoxyribonuclease, Deoxyribonucleate 5′-oligonucleotido-hydrolase
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352204
Javasolt termékek
biológiai forrás
bovine pancreas
Minőségi szint
form
lyophilized
kiszerelés
pkg of 100 mg
gyártó/kereskedő neve
Roche
technika/technikák
DNA extraction: suitable
optimális pH
~7.0
tárolási hőmérséklet
2-8°C
Általános leírás
Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.
Alkalmazás
Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-
- The isolation of cells from lung, skin and tumor samples
- Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †
Egység definíció
One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.
Elkészítési megjegyzés
Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Resp. Sens. 1 - Skin Sens. 1
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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