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P3563

Sigma-Aldrich

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween® 20

Szinonimák:

1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

10 PKG
29 900,00 Ft
5 X 10 PKG
104 000,00 Ft

29 900,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben
Összes fotó(1)

Méret kiválasztása

Nézet módosítása
10 PKG
29 900,00 Ft
5 X 10 PKG
104 000,00 Ft

About This Item

UNSPSC kód:
41105319
eCl@ss:
32129211
NACRES:
NA.25

29 900,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben

Terméknév

Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4,

Forma

powder

Minőségi szint

200

oldhatóság

water: soluble, clear, colorless (1 pouch dissolved in 1L)

alkalmazás(ok)

cell analysis
diagnostic assay manufacturing

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Általános leírás

Phosphate buffered saline with TWEEN® 20, or PBS-T, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN 20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBS-T buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.

Alkalmazás

Phosphate buffered saline has been used in:

  • western blotting
  • enzyme-linked immunosorbent assay (ELISA)[1]
  • telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)[2][3][4]
  • cell isolation[5]

Kiszerelés

Foil pouches

Feloldás

Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.

Egyéb megjegyzések

For additional information on our range of Biochemicals, please complete this form.

Jogi információk

TWEEN is a registered trademark of Croda International PLC

Tárolási osztály kódja

13 - Non Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000393719
0000391849
0000342977
0000379944
0000371889

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The alternative lengthening of telomeres phenotype in breast carcinoma is associated with HER-2 overexpression
Subhawong A P, et al.
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Liu Z, et al.
Oncology Letters, 11(3), 1829-1834 (2016)
Shorter telomeres in luminal B, HER-2 and triple-negative breast cancer subtypes
Heaphy C M, et al.
Modern Pathology, 24(2), 194-194 (2011)
Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining
Meeker A K, et al.
The American Journal of Pathology, 160(4), 1259-1268 (2002)
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Protocols

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. I am looking for the expiry date of P3563, lot number SLCP6390 please and thanks. It is not printed on the COA received with the item.

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

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