93363

Trizma^® hydrochloride

BioUltra, for molecular biology, ≥99.0% (AT)

• Szinonimák: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Lineáris képlet: NH₂C(CH₂OH)₃ · HCl
• CAS-szám: 1185-53-1
• Molekulatömeg: 157.60
• Beilstein: 3675235
• EC-szám: 214-684-5
• MDL-szám: MFCD00012590
• UNSPSC kód: 12161700
• PubChem Substance ID: 57653541
• NACRES: NA.25

Tulajdonságok

• grade: for molecular biology
• Minőségi szint: 200
• termékcsalád: BioUltra
• Teszt: ≥99.0% (AT)
• Forma: powder or crystals
• szennyeződések: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• izzítási maradék: ≤0.2% (as SO₄)
• veszteség: ≤0.2% loss on drying, 110 °C
• pH: 2.5-4.0 (25 °C, 4 M in H₂O)
• hasznos pH-tartomány: 7.0-9.0
• pKa (25 °C): 8.1
• mp: 150-152 °C
• oldhatóság: H₂O: 4 M at 20 °C, clear, colorless
• anion nyomok: sulfate (SO₄^2-): ≤50 mg/kg
• kation nyomok: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 4 M in H₂O
• UV-elnyelés: λ: 260 nm A_max: 0.12; λ: 280 nm A_max: 0.08
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• Nemzetközi kémiai azonosító kulcs: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Leírás

Alkalmazás

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.

Egyéb megjegyzések

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Component of extraction buffer e.g. phenol extraction of DNA or RNA; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE.

Jogi információk

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: dust mask type N95 (US), Eyeshields, Gloves