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XTG9-RO

Roche

X-tremeGENE™ 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(6)

About This Item

UNSPSC kód:
41106502
NACRES:
NA.55

grade

for molecular biology

Minőségi szint

100

Forma

liquid (aqueous solution)

használat

 mL (suitable for 165 transfections)

kiszerelés

pkg of 0.4 mL (06365779001)
pkg of 1.0 mL (06365787001)
pkg of 5 × 1.0 mL (06365809001)

gyártó/kereskedő neve

Roche

technika/technikák

transfection: suitable

tárolási hőmérséklet

2-8°C

Related Categories

Általános leírás

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.

Alkalmazás

X-tremeGENE™ 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE™ 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:
  • Expression of recombinant proteins for functional analysis.
  • Physiological studies of metabolic pathways.
  • Analysis of regulatory sequences using reporter gene assays.
  • Gene expression assays.
  • Cancer research studies.
  • Target evaluation.

Tulajdonságok és előnyök

  • Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
  • Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
  • Avoid time-consuming optimization procedures in commonly used cell lines.

Minőség

Each lot of X-tremeGENE™ 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE™ 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Fizikai forma

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Jogi információk

X-tremeGENE is a trademark of Roche

kapcsolódó termék

Product No.
Leírás
Árazás

D5796

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

R8758

RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

NA0300

GenElute™ HP Plasmid Maxiprep Kit, sufficient for 10 purifications

NA0400

GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit, sufficient for 10 preparations

PP2393

Mammalian FLAG® Tag Vector Set, plasmid vectors for molecular cloning

XTGHP-RO

X-tremeGENE™ HP DNA Transfection Reagent, High-performance polymer reagent for transfecting many cell lines

SITRAN-RO

X-tremeGENE™ siRNA Transfection Reagent, Polymer reagent for delivering siRNA to common cell lines

Piktogramok

FlameExclamation mark
GHS02,GHS07

Figyelmeztetés

Danger

Figyelmeztető mondatok

H225 - H319

Veszélyességi osztályok

Eye Irrit. 2 - Flam. Liq. 2

Tárolási osztály kódja

3 - Flammable liquids

WGK

WGK 1

Lobbanási pont (F)

334.4 °F

Lobbanási pont (C)

168 °C

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
44348000
34569800
33315600
27238700
25583000

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COA keresése

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Dokumentumtár megtekintése

Leonardo Freire-de-Lima et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(43), 17690-17695 (2011-10-19)
The process termed "epithelial-mesenchymal transition" (EMT) was originally discovered in ontogenic development, and has been shown to be one of the key steps in tumor cell progression and metastasis. Recently, we showed that the expression of some glycosphingolipids (GSLs) is
L C Costantini et al.
Neuroscience, 89(2), 505-513 (1999-03-17)
To investigate the role of neurotrophins in the initial formation of striatal patch versus matrix, the spatial and temporal expression of trkB receptors was examined using immunohistochemistry. Polyclonal antibodies, against the C-terminus or the tyrosine kinase domain, revealed trkB-immunoreactive cells
Piroon Jenjaroenpun et al.
BMC genomics, 10 Suppl 3, S9-S9 (2010-01-09)
DNA triplexes can naturally occur, co-localize and interact with many other regulatory DNA elements (e.g. G-quadruplex (G4) DNA motifs), specific DNA-binding proteins (e.g. transcription factors (TFs)), and micro-RNA (miRNA) precursors. Specific genome localizations of triplex target DNA sites (TTSs) may
Daniel F Comiskey et al.
Nucleic acids research, 43(8), 4202-4218 (2015-04-08)
Genotoxic stress induces alternative splicing of the oncogene MDM2 generating MDM2-ALT1, an isoform attributed with tumorigenic properties. However, the mechanisms underlying this event remain unclear. Here we explore MDM2 splicing regulation by utilizing a novel minigene that mimics endogenous MDM2
Jean Guillon et al.
Journal of enzyme inhibition and medicinal chemistry, 18(2), 147-153 (2003-08-29)
The syntthesis of new N-propargyl-3-pyrrol-1-ylindanamine derivatives, analogues of rasagiline, is described in ten steps starting from the corresponding arylaldehydes via the corresponding cis-3-pyrrol-1-ylindanamines. The cis-configuration of some intermediates has been established using X-ray analysis and NOE experiments. The new N-propargyl-3-pyrrol-1-ylindanamine
View All Related Papers

Cikkek

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocols

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ HP DNS transzfekciós reagens protokollja

Sejtek előkészítése a transzfekcióhoz A sejteket kb. 24 órával a transzfekció előtt lemezelje le, és győződjön meg arról, hogy a sejtek optimális koncentrációban vannak (70-90%-os konfluencia).

Összes mutatása

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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