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CAPHOS

Sigma-Aldrich

Calcium Phosphate Transfection Kit

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

Szinonimák:

Gene delivery

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About This Item

EC-szám:
233-140-8
UNSPSC kód:
12352200
NACRES:
NA.85

grade

for molecular biology

Minőségi szint

200

Forma

solution

használat

 kit sufficient for 160 transfections (6 cm dishes)
 kit sufficient for 400 transfections (3.5 cm dishes)
 kit sufficient for 80 transfections (10 cm dishes)

technika/technikák

transfection: suitable

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

Általános leírás

Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.

Alkalmazás

Calcium Phosphate Transfection Kit has been used:
  • to enable transfection
  • to transfect Hek293T cells
  • to transfect H29D cells

Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:

BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D

Tulajdonságok és előnyök

  • Suitable for transient and stable transfection
  • Reproducible for a wide range of cell types
  • Widely referenced
  • Inexpensive

Komponensek

The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)

Elv

The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.

Egyéb megjegyzések

The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.

Piktogramok

Exclamation mark
GHS07

Figyelmeztetés

Warning

Figyelmeztető mondatok

H319

Óvintézkedésre vonatkozó mondatok

P264 - P280 - P305 + P351 + P338 - P337 + P313

Veszélyességi osztályok

Eye Irrit. 2

Tárolási osztály kódja

12 - Non Combustible Liquids

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000235326
069M4126V
11190141
037M4170V
11161104

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COA keresése

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HEPES Buffered Saline Solution Hepes BSS (30 mM), 250 ml

Sigma-Aldrich

C-40020

HEPES Buffered Saline Solution

Insect GeneJuice® Transfection Reagent Proprietary liposome transfection reagent optimized for maximal transfection efficiency of Sf9 insect cells for baculovirus protein expression.

Sigma-Aldrich

71259

Insect GeneJuice® Transfection Reagent

Denise A Carbonaro et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 22(3), 607-622 (2013-11-22)
Gene transfer into autologous hematopoietic stem cells by γ-retroviral vectors (gRV) is an effective treatment for adenosine deaminase (ADA)-deficient severe combined immunodeficiency (SCID). However, current gRV have significant potential for insertional mutagenesis as reported in clinical trials for other primary
Paolo Conrotto et al.
Molecular oncology, 5(6), 527-537 (2011-09-02)
The transcription factor SOX11 is a novel diagnostic marker for mantle cell lymphoma (MCL), distinguishing this aggressive tumor from potential simulators. Recent data also show that the level of SOX11 correlates to in vitro growth properties in MCL, as well
Use of retroviral-mediated gene transfer to deliver and test function of chimeric antigen receptors in human T-cells
Parente-Pereira A C, et al.
Journal of biological methods, 1(2), e7-e7 (2014)
Virginie Mournetas et al.
Journal of biological methods, 3(4), e55-e55 (2016-10-04)
Gene silencing techniques, including RNA interference methodologies, are widely used in reverse genetics to study the role of specific genes in biological processes. RNA interference has become easier to implement thanks to the RNAi Consortium (TRC), which has developed libraries
A new chimeric protein represses HIV-1 LTR-mediated expression by DNA methylase
Martinez-Colom A, et al.
Antiviral research, 98(3), 394-400 (2013)
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Cikkek

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocols

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Related Content

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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