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L3037

Sigma-Aldrich

Escort™ III Transfection Reagent

Lipid reagent for transfecting sensitive and primary cells

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

UNSPSC kód:
41106502
NACRES:
NA.25

grade

for molecular biology

Minőségi szint

200

Forma

liquid (aqueous solution)

használat

1 mL sufficient for 250-1000 transfections

koncentráció

1 mg/mL

technika/technikák

transfection: suitable

tárolási hőmérséklet

2-8°C

Általános leírás

Escort™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.

Alkalmazás

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort™ III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort™ III:

A549
C2C12 myotubes
Cardiomyocytes (rat)
COS-7
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
HepG2
HeLa
Jurkat
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
NIH3T3
PC-12
Retinal Neurons (rat)
Tracheobronchial cells (sheep)

Tulajdonságok és előnyök

  • Suitable for stable and transient transfection
  • Optimized for a wide variety of primary cells
  • Low toxicity
  • Compatible with both serum and serum-free transfection protocols
  • Ideal for PC-12 cells

Komponensek

Escort™ III formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort™ III is confidential.

Vigyázat

Do not freeze.

Elv

A stable complex is formed when Escort™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Jogi információk

Escort is a trademark of Sigma-Aldrich Co. LLC

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
SLCQ5069
SLCP3114
SLCR4420
SLCF5368
SLBV8324

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COA keresése

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Dokumentumtár megtekintése

Patrick Orth et al.
Molecular biotechnology, 38(2), 137-144 (2008-01-26)
The aim of the present study was to evaluate the efficacy of novel nonviral gene delivery systems in cells of musculoskeletal origin. Primary cultures of lapine skeletal muscle cells, lapine articular chondrocytes, human cells from fibrous dysplasia and cell lines
Tomás C O'Riordan et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 292(4), R1613-R1620 (2006-12-16)
The development and application of a methodology for measurement of oxygen within single mammalian cells are presented, which employ novel macromolecular near infrared (NIR) oxygen probes based on new metalloporphyrin dyes. The probes, which display optimal spectral characteristics and sensitivity
Lixian Liu et al.
Frontiers in cell and developmental biology, 9, 634242-634242 (2021-03-12)
The mitogen-inducible gene 6 (MIG6) is an adaptor protein widely expressed in vascular endothelial cells. However, it remains unknown thus far whether it plays a role in angiogenesis. Here, using comprehensive in vitro and in vivo model systems, we unveil
Inmaculada Navarro-Lérida et al.
Journal of cell science, 117(Pt 9), 1687-1697 (2004-04-13)
Several cell types express inducible nitric oxide synthase (NOS2) in response to exogenous insults such as bacterial lipopolysaccharide (LPS) or proinflammatory cytokines. For instance, muscular cells treated with LPS and interferon gamma (IFN-gamma) respond by increasing the mRNA and protein
Thomas Iskratsch et al.
The Journal of cell biology, 191(6), 1159-1172 (2010-12-15)
Members of the formin family are important for actin filament nucleation and elongation. We have identified a novel striated muscle-specific splice variant of the formin FHOD3 that introduces a casein kinase 2 (CK2) phosphorylation site. The specific targeting of muscle

Cikkek

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocols

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Related Content

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

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