Összes fotó(1)
Fontos dokumentumok
RNASEA-RO
Roche
RNase A
from bovine pancreas
Szinonimák:
Rnase
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Javasolt termékek
biológiai forrás
bovine pancreas
Minőségi szint
Forma
dry powder
specifikus aktivitás
50 U/mg (+25°C, RNA as substrate)
molekulatömeg
Mr = 13,700
kiszerelés
pkg of 100 mg (10109169001)
pkg of 25 mg (10109142001)
gyártó/kereskedő neve
Roche
parameter
65 °C optimum reaction temp.
technika/technikák
flow cytometry: suitable
optimális pH
7.0-7.5
tárolási hőmérséklet
2-8°C
Általános leírás
Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.
Alkalmazás
- For analytical purposes
- Isolation of DNA (for this purpose, RNase A should be boiled)
- For cell cycle analysis by flow cytometry and propidium iodide (PI) staining
Biokémiai/fiziológiai hatások
RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding′ property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.
Egység definíció
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Feloldás
RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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