MAB5308
Anti-BACE Antibody, CT, clone 61-3E7
clone 61-3E7, Chemicon®, from mouse
Szinonimák:
ASP2, BACE1, Beta Secretase, beta-Site APP Cleaving Enzyme
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
61-3E7, monoclonal
faj reaktivitás
primate, mouse, human, rat
gyártó/kereskedő neve
Chemicon®
technika/technikák
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG1
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... BACE1(23621)
Általános leírás
Alzheimer′s disease (AD) is characterized by the progressive formation in the brain of insoluble amyloid plaques and vascular deposits consisting of the 4-kD amyloid b-peptide (Ab). Ab generation is initiated by proteolytic cleavage of the amyloid precursor protein (APP) at the N-terminal of Ab by b-secretase. The Ab peptide is then released by proteolytic cleavage at its C-terminus by g-secretase. Because both these proteases are prime candidates for therapeutic intervention, an intense search has been underway to identify these two enzymes.A human transmembrane aspartic-protease (Asp2), referred to as BACE, has been characterized and shown to have all the properties of b-secretase. Four groups in all have now confirmed that BACE (or Asp2) is a convincing candidate for b-secretase.
BACE is an N-glycosylated integral membrane aspartyl protease with Mr=70 kDa. Mature BACE is produced from the immature form through a series of post-translational proteolytic cleavages and glycosylation. Sequence analysis has revealed that the immature form of BACE contains an N-terminal signal sequence (residues 1-21) followed by a large catalytic domain, a single transmembrane domain (residues 461-477), and a short cytoplasmic domain (residues 478-501). The signal sequence (1-21) is cleaved from the immature form by a signal peptidase located in the endoplasmic reticulum (ER), yielding the proBACE protein (Mr=75 kDa) which starts at residue 22. The proBACE protein is modified by cleavage of 24 N-terminal residues (aa 22-45), producing the mature BACE protein.
BACE is an N-glycosylated integral membrane aspartyl protease with Mr=70 kDa. Mature BACE is produced from the immature form through a series of post-translational proteolytic cleavages and glycosylation. Sequence analysis has revealed that the immature form of BACE contains an N-terminal signal sequence (residues 1-21) followed by a large catalytic domain, a single transmembrane domain (residues 461-477), and a short cytoplasmic domain (residues 478-501). The signal sequence (1-21) is cleaved from the immature form by a signal peptidase located in the endoplasmic reticulum (ER), yielding the proBACE protein (Mr=75 kDa) which starts at residue 22. The proBACE protein is modified by cleavage of 24 N-terminal residues (aa 22-45), producing the mature BACE protein.
Egyediség
Reacts with BACE (beta-site APP Cleaving Enzyme). Shows no reactivity to BACE2 by Western blot.
Immunogen
Epitope: C-terminus
Synthetic peptide corresponding to the C-terminus of human BACE.
Alkalmazás
Detect BACE using this Anti-BACE Antibody, C-terminus, clone 61-3E7 validated for use in IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Western blotting: 1 μg/mL; recognizes pro and mature forms: ~60-75kDa on reducing westerns. BACE is N-terminally glycosylated which causes the wide size range.
Immunohistochemistry on paraformaldehyde fixed tissues from human, rat, mouse and monkey.
Immunocytochemistry on cells expressing BACE. Acetone or methanol fixation preferred; 4% PFA 5′, RT followed by 0.1% triton X-100 1 hour, can also be used. 1:200-1:500 is recommended, optimization is necessary.
Immunoprecipitation:
Optimal working dilutions must be determined by end user.
Immunohistochemistry on paraformaldehyde fixed tissues from human, rat, mouse and monkey.
Immunocytochemistry on cells expressing BACE. Acetone or methanol fixation preferred; 4% PFA 5′, RT followed by 0.1% triton X-100 1 hour, can also be used. 1:200-1:500 is recommended, optimization is necessary.
Immunoprecipitation:
Optimal working dilutions must be determined by end user.
Cél megnevezése
~ 60-75 kDa
Fizikai forma
Format: Purified
Protein A purified
Purified immunoglobulin. Liquid. Buffer = 0.02M Sodium Phosphate, 0.25M NaCl with 0.1% sodium azide.
Tárolás és stabilitás
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analízis megjegyzés
Control
Brain
Brain
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Sindhu Ramesh et al.
PloS one, 13(1), e0190350-e0190350 (2018-01-13)
Honokiol (poly-phenolic lignan from Magnolia grandiflora) is a Sirtuin-3 (SIRT3) activator which exhibit antioxidant activity and augment mitochondrial functions in several experimental models. Modern evidence suggests the critical role of SIRT3 in the progression of several metabolic and neurodegenerative diseases.
BACE1 and BACE2 enzymatic activities in Alzheimer's disease.
Ahmed, RR; Holler, CJ; Webb, RL; Li, F; Beckett, TL; Murphy, MP
Journal of Neurochemistry null
Li Zhu et al.
The Journal of biological chemistry, 288(44), 32050-32063 (2013-09-21)
Recent studies link synaptojanin 1 (synj1), the main phosphoinositol (4,5)-biphosphate phosphatase (PI(4,5)P2-degrading enzyme) in the brain and synapses, to Alzheimer disease. Here we report a novel mechanism by which synj1 reversely regulates cellular clearance of amyloid-β (Aβ). Genetic down-regulation of
Beta-secretase activity increases with aging in human, monkey, and mouse brain.
Fukumoto, H; Rosene, DL; Moss, MB; Raju, S; Hyman, BT; Irizarry, MC
The American Journal of Pathology null
Dynamin 1 regulates amyloid generation through modulation of BACE-1.
Zhu, L; Su, M; Lucast, L; Liu, L; Netzer, WJ; Gandy, SE; Cai, D
Testing null
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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