MAB2247
Anti-Integrin β1 Antibody, clone 12G10
clone 12G10, Chemicon®, from mouse
Szinonimák:
Integrin beta-1, CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
12G10, monoclonal
application:
ELISA
FACS
IP
WB
cell culture
FACS
IP
WB
cell culture
faj reaktivitás:
human
technika/technikák:
ELISA: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
21
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
12G10, monoclonal
faj reaktivitás
human
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG1κ
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... ITGB1(3688)
Általános leírás
Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
Egyediség
MAB2247 recognizes the human beta1 integrin. This antibody has been shown to recognize a ligand-induced binding site, with antibody binding increasing in the presence of a fibronectin ligand. This antibody also enhances alpha 5 - beta 1 fibronectin interactions. MAB2247 does not cross-react with mouse or rat samples.
Immunogén
Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.
Alkalmazás
Anti-Integrin β1 Antibody, clone 12G10 detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IP, FUNC, CULT & WB.
Flow cytometry: 1:25-1:50
ELISA : 10 μg/mL
Western Blot: known to react with human beta 1 integrin subunit under non-reduced conditions only {Mould, 1995}.
Stimulatory to alpha5beta1 interaction with fibronectin {Mould, 1995}.
Optimal working dilutions must be determined by end user.
ELISA : 10 μg/mL
Western Blot: known to react with human beta 1 integrin subunit under non-reduced conditions only {Mould, 1995}.
Stimulatory to alpha5beta1 interaction with fibronectin {Mould, 1995}.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Cél megnevezése
88 kDa
Fizikai forma
Format: Purified
Protein A purified
Purified immunoglobulin. Liquid in phosphate buffer saline, with 0.1% sodium azide.
Tárolás és stabilitás
Maintain refrigerated at 2–8°C in undiluted aliquots for up one month. For long term storage maintain at -20°C for up to 12 months.
Analízis megjegyzés
Control
Tonsil, human skin tissue
Tonsil, human skin tissue
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Magdalene Michael et al.
The Journal of investigative dermatology, 134(3), 773-782 (2013-09-13)
Bullous pemphigoid antigen 1 (BPAG1-e, also known as BP230) is a member of the plakin family of hemidesmosome cytoskeletal linker proteins that is encoded by an isoform of the dystonin (DST) gene. Recently, we reported two unrelated families with homozygous
Catherine T Choy et al.
Endocrine-related cancer, 21(1), 85-99 (2013-11-06)
Anosmin-1, encoded by the KAL1 gene, is an extracellular matrix (ECM)-associated protein which plays essential roles in the establishment of olfactory and GNRH neurons during early brain development. Loss-of-function mutations of KAL1 results in Kallmann syndrome with delayed puberty and
Dhaarmini Rajshankar et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(11), 4429-4444 (2012-07-26)
IL-1β is a prominent proinflammatory cytokine that mediates degradation of extracellular matrix proteins through increased expression of matrix metalloproteinases, which involves a signaling pathway in adherent cells that is restricted by focal adhesions. Currently, the mechanism by which IL-1β affects
Zhenwei Lu et al.
eLife, 5 (2016-12-09)
Integrins are transmembrane receptors composed of α and β subunits. Although most integrins contain β1, canonical activation mechanisms are based on studies of the platelet integrin, αIIbβ3. Its inactive conformation is characterized by the association of the αIIb transmembrane and
H T Le et al.
Molecular human reproduction, 24(10), 495-509 (2018-08-21)
Does A Disintegrin And Metalloproteinase 8 (ADAM8) control extravillous trophoblast (EVT) differentiation and migration in early human placental development? ADAM8 mRNA preferentially localizes to invasive HLA-G-positive trophoblasts, associates with the acquirement of an EVT phenotype and promotes trophoblast migration through
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