MAB1997
Anti-Integrin β1 Antibody, clone MB1.2
clone MB1.2, Chemicon®, from rat
Szinonimák:
CD29 antigen, Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, fibronectin receptor beta subunit, integrin VLA-4 beta subunit, integrin beta 1, integrin beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rat
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
MB1.2, monoclonal
faj reaktivitás
mouse, human
faj reaktivitás (homológia által előrejelzett)
rat
kiszerelés
antibody small pack of 25 μg
gyártó/kereskedő neve
Chemicon®
technika/technikák
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG2κ
NCBI elérési szám
UniProt elérési szám
kiszállítva
ambient
tárolási hőmérséklet
2-8°C
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... ITGB1(3688)
Általános leírás
Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
Egyediség
Reacts with β1 subunit of VLA (β1) integrins
Immunogen
Mouse Integrin beta-1, as per (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132). Mouse Integrin beta-1, as per (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).
Alkalmazás
Anti-Integrin β1 Antibody, clone MB1.2 is an antibody against Integrin β1 for use in FC, IH, IP & WB.
Immunoprecipitation:
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.
Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.
Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.
Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.
Optimal working dilutions must be determined by end user.
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.
Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.
Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.
Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.
Optimal working dilutions must be determined by end user.
Minőség
Routinely evaluated by Western Blot on C2C12 lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected integrin β1 on 10 μg of C2C12 lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected integrin β1 on 10 μg of C2C12 lysate.
Cél megnevezése
~88 kDa (non-glycosylated) and ~130 kDa ( glycosylated).
Kapcsolódás
Replaces: 04-1109
Fizikai forma
Format: Purified
Purified rat monoclonal IgG2κ liquid in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% NaN3
Analízis megjegyzés
Control
Human OVCAR5 cell line whole cell lysate
C2C12 lysate.
Human OVCAR5 cell line whole cell lysate
C2C12 lysate.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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javasolt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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