ABN76

Anti-Neurofilament H (200 kDa) Antibody

rabbit polyclonal

• Szinonimák: Neurofilament heavy polypeptide, Neurofilament H
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• Terméknév: Anti-Neurofilament H (200 kDa) Antibody, from rabbit, purified by affinity chromatography
• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• tisztítva: affinity chromatography
• faj reaktivitás: mouse, human, rat, bovine
• technika/technikák: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI elérési szám: NP_066554.2
• UniProt elérési szám: P12036
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: bovine ... Nefh(528842) ; human ... NEFH(4744) ; mouse ... Nefh(380684) ; rat ... Nefh(24587)

Leírás

Általános leírás

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas, carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung. For moreinformation, see the Nervous System Cell Type Specific Marker chart on the Millipore website.

Immunogén

GST-tagged recombinant protein corresponding to bovine Neurofilament H.

Alkalmazás

Immunohistochemistry Analysis: A 1 µg/mL dilution from a representative lot detected Neurofilament H in normal rat cerebellum tissue.

This Anti-Neurofilament H (200 kDa) Antibody is validated for use in WB, IH(P) for the detection of Neurofilament H (200 kDa).

Minőség

Evaluated by Western Blot in bovine cerebellum tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Neurofilament H in bovine cerebellum tissue lysate.

Cél megnevezése

~200 kDa observed

Kapcsolódás

Replaces: AB1982

Analízis megjegyzés

Control
Bovine cerebellum tissue lysate

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable