As it shows in the link below, MAB318-AF488 has been used for detection of tyrosine hydroxylase in SCC196. Theoretically AB152 should work but it has not been tested in house.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/130/252/scc196ds.pdf
AB152
Anti-Tyrosine Hydroxylase Antibody
CHEMICON®, rabbit polyclonal
Szinonimák:
Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase, tyrosine hydroxylase, TH
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Méret kiválasztása
100 μL
201 000,00 Ft
201 000,00 Ft
Szállítás becsült dátuma2025. február 11.részletek
Az adott célhoz egy rekombináns, tartósítószer-mentes antitest elérhető. Próbálja meg ezt: ZMS1033
Vásárlás csomagban
Megrendelés igénylése nagy tételben
Összes fotó(9)
Méret kiválasztása
Nézet módosítása
100 μL
201 000,00 Ft
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
201 000,00 Ft
Szállítás becsült dátuma2025. február 11.részletek
Az adott célhoz egy rekombináns, tartósítószer-mentes antitest elérhető. Próbálja meg ezt: ZMS1033
Vásárlás csomagban
Megrendelés igénylése nagy tételben
Javasolt termékek
Terméknév
Anti-Tyrosine Hydroxylase Antibody, Chemicon®, from rabbit
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
human, mollusk, feline, ferret, Aplysia (marine mollusc), rat, squid, Drosophila, mouse
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Általános leírás
Tyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Egyediség
Anti-Tyrosine Hydroxylase Antibodies react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin-embedded human tissue
Tyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
Immunogén
Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
Alkalmazás
Anti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.
Immunohistochemistry:
A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.
Immunofluorescence:
A previous lot of this antibody was used at 1:100-1:1,000 dilution.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
ELISA:
A previous lot of this antibody was used in ELISA.
Optimal dilutions must be determined by the end user.
A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.
Immunofluorescence:
A previous lot of this antibody was used at 1:100-1:1,000 dilution.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
ELISA:
A previous lot of this antibody was used in ELISA.
Optimal dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neurotransmitters & Receptors
Neuronal & Glial Markers
Minőség
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
Cél megnevezése
62 kDa
Fizikai forma
Affinity purified
Purified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.
Tárolás és stabilitás
Maintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
Analízis megjegyzés
Control
POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
NEGATATIVE CONTROL: Liver.
POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
NEGATATIVE CONTROL: Liver.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.
Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.
Fedezze fel az antitestekkel való munka alapjait, beleértve a szerkezetre, az osztályokra és a normál immunglobulin-tartományokra vonatkozó technikai információkat.
Az immunfluoreszcencia antitesthez kötött fluoreszcens molekulákat használ a fehérjék lokalizációjára, a módosítások megerősítésére és a fehérjekomplexek vizualizálására.
Protocols
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
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Greetings, I would like to know if this Antibody can be used for immune cytochemistry procedure with dopaminergic cell line N27-A
1 answer-
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