AB1784P
Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain
Chemicon®, from rabbit
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity purified immunoglobulin
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
rat
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... DRD1(1812)
Egyediség
Dopamine D1A Receptor (D1R). The rat D1A receptor is a 446 amino acid, G-protein coupled transmembrane receptor protein (Monsma, 1990; Zhou, 1990; Zhou, 1992; O′Dowd, 1990). The immunogen sequence has 88% sequence similarity with human and 77% with porcine.
Immunogen
A nine amino acid peptide corresponding to the 4th extracellular domain of the rat D1A receptor. The immunogen is absolutely conserved in the mouse D1A receptor and shows 88% homology with human and 77% with porcine and bovine D1A receptors. The peptide shows no significant sequence similarity with other dopamine receptors (D1B, D2-D5).
Epitope: 4th Extracellular Domain
Alkalmazás
Detect Dopamine D1A Receptor using this Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain validated for use in ELISA, IH & WB.
Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed tissue sections. D1R has been localized in various regions of the rat brain (Huang, 1992; O′Connel, 1995)
Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang, 1992; O′Connel, 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang, 1992; O′Connel, 1995) that is present in relatively small amounts.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of control peptide per well.
The immunogen peptide is available (Cat. No. AG266) for use in ELISA or antibody blocking studies. AG266 is not recommended for use as a western blot positive control.
Optimal working dilutions must be determined by end user.
Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang, 1992; O′Connel, 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang, 1992; O′Connel, 1995) that is present in relatively small amounts.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of control peptide per well.
The immunogen peptide is available (Cat. No. AG266) for use in ELISA or antibody blocking studies. AG266 is not recommended for use as a western blot positive control.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Fizikai forma
Affinity purified immunoglobulin. Liquid in PBS with 0.2% BSA and 0.05% sodium azide.
Tárolás és stabilitás
Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
Analízis megjegyzés
Control
POSITIVE CONTROL: rat striatal membranes (western blot)
POSITIVE CONTROL: rat striatal membranes (western blot)
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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