MAB3034
Anti-DNA Antibody, single stranded, clone 16-19
clone 16-19, Chemicon®, from mouse
Szinonimák:
Single-Stranded DNA Antibody
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
16-19, monoclonal
faj reaktivitás (homológia által előrejelzett)
all
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
izotípus
IgG2aκ
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Egyediség
Single stranded DNA (ssDNA) (poly-deoxythymidine). Shows 90% reactivity to ssDNA and 12% reactivity to dsDNA.
ANTIGEN BINDING PROFILE:
Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53
a) Native calf thymus DNA
b) Heat-denatured calf thymus DNA
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
BINDING ASSAY
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ISOELECTRIC POINT = 8.3
ANTIGEN BINDING PROFILE:
Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53
a) Native calf thymus DNA
b) Heat-denatured calf thymus DNA
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
BINDING ASSAY
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ISOELECTRIC POINT = 8.3
Immunogen
Epitope: single stranded DNA
Alkalmazás
ELISA
This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus.
Optimal working dilutions must be determined by end user.
This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus.
Optimal working dilutions must be determined by end user.
Anti-DNA Antibody, single stranded, clone 16-19 is an antibody against DNA for use in ELISA.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Cell Cycle, DNA Replication & Repair
Apoptosis - Additional
Cell Cycle, DNA Replication & Repair
Fizikai forma
0.1 M Tris-Glycine (pH 7.4), 150 nM NaCl with 0.05% sodium azide.
Format: Purified
Tárolás és stabilitás
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Cyclin-dependent kinase regulates the length of S phase through TICRR/TRESLIN phosphorylation.
Sansam, CG; Goins, D; Siefert, JC; Clowdus, EA; Sansam, CL
Genes & Development null
Genome instability and transcription elongation impairment in human cells depleted of THO/TREX.
Dominguez-Sanchez, MS; Barroso, S; Gomez-Gonzalez, B; Luna, R; Aguilera, A
PLoS Genetics null
Anne Fernandez-Vidal et al.
Nature communications, 5, 4285-4285 (2014-07-06)
Although DNA polymerase θ (Pol θ) is known to carry out translesion synthesis and has been implicated in DNA repair, its physiological function under normal growth conditions remains unclear. Here we present evidence that Pol θ plays a role in
Therese Wilhelm et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(2), 763-768 (2013-12-19)
Homologous recombination deficient (HR(-)) mammalian cells spontaneously display reduced replication fork (RF) movement and mitotic extra centrosomes. We show here that these cells present a complex mitotic phenotype, including prolonged metaphase arrest, anaphase bridges, and multipolar segregations. We then asked
Vincent Gaggioli et al.
The Journal of cell biology, 204(4), 507-522 (2014-02-19)
Cyclin-dependent kinase (CDK) plays a vital role in proliferation control across eukaryotes. Despite this, how CDK mediates cell cycle and developmental transitions in metazoa is poorly understood. In this paper, we identify orthologues of Sld2, a CDK target that is
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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