AB144P

Anti-Choline Acetyltransferase Antibody

CHEMICON^®, goat polyclonal

• Szinonimák: Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• product name: Anti-Choline Acetyltransferase Antibody, Chemicon^®, from goat
• biológiai forrás: goat
• Minőségi szint: 100
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• tisztítva: affinity chromatography
• faj reaktivitás: human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken
• gyártó/kereskedő neve: Chemicon^®
• technika/technikák: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• NCBI elérési szám: NM_020549.3
• UniProt elérési szám: P28329
• kiszállítva: dry ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: chicken ... Chat(395314) ; human ... CHAT(1103) ; mouse ... Chat(12647) ; rat ... Chat(290567) ; rhesus monkey ... Chat(709977)

Leírás

Általános leírás

Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.

Egyediség

Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.

Immunogen

Human placental enzyme.

Alkalmazás

Anti-Choline Acetyltransferase Antibody has been used in:

• immunohistochemical staining
• western blotting
• immunocytochemistry

Immunohistochemistry:
A previous lot of this antibody was used at 1:100.

Optimal working dilutions and protocols must be determined by end user.

Biokémiai/fiziológiai hatások

Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.

Minőség

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.

Cél megnevezése

70/74 kDa

Fizikai forma

Affinity purified
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

Tárolás és stabilitás

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.

Analízis megjegyzés

Control
Human placenta lysates, rat forebrain/rostral hypothalamus.

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Research Category:
Neuroscience

Research Sub Category:
Neurotransmitters & Receptors

Neuronal & Glial Markers

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable