AB1542
Anti-Tyrosine Hydroxylase Antibody
CHEMICON®, sheep polyclonal
Szinonimák:
Tyrosine 3-hydroxylase, Tyrosine 3-hydroxylase, tyrosine hydroxylase
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
IHC
WB
WB
faj reaktivitás:
rat, mouse
technika/technikák:
immunohistochemistry: suitable
western blot: suitable
western blot: suitable
citations:
339
Javasolt termékek
Terméknév
Anti-Tyrosine Hydroxylase Antibody, Chemicon®, from sheep
biológiai forrás
sheep
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
rat, mouse
faj reaktivitás (homológia által előrejelzett)
mammals
gyártó/kereskedő neve
Chemicon®
technika/technikák
immunohistochemistry: suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... TH(7054)
Általános leírás
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Egyediség
Tyrosine Hydroxylase (TH, Tyrosine Monooxygenase). Cross-reacts with all mammalian forms tested to date and some non-mammalian forms.
Immunogén
Native tyrosine hydroxylase from rat pheochromocytoma (J. Biol. Chem.,1982, 257:9416-9423).
Alkalmazás
Anti-Tyrosine Hydroxylase Antibody is an antibody against Tyrosine Hydroxylase for use in IH & WB.
Immunohistochemistry(peroxidase): A previous lot of this antibody was used at 1:1,000 dilution. The antibody gives specific labeling of noradrenergic axons in primate cerebral cortex (Brain Res., 1989, 500:313-324.).
Note: It is important that you use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.
Optimal working dilutions must be determined by the end user.
Note: It is important that you use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neurotransmitters & Receptors
Neuronal & Glial Markers
Minőség
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of mouse brain lysates.
Cél megnevezése
~60 kDa
Fizikai forma
ImmunoAffinity Purified
Purified sheep polyclonal in buffer containing 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Tárolás és stabilitás
Stable for 6 months at -20 to -80°C in undiluted aliquots from date of receipt. Store in a NON-self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analízis megjegyzés
Control
Positive: brain (corpus striatum) and adrenal gland. Negative: liver.
Positive: brain (corpus striatum) and adrenal gland. Negative: liver.
Egyéb megjegyzések
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Analitikai tanúsítványok (COA)
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Az ügyfelek ezeket is megtekintették
Selective optogenetic activation of rostral ventrolateral medullary catecholaminergic neurons produces cardiorespiratory stimulation in conscious mice.
Abbott, SB; DePuy, SD; Nguyen, T; Coates, MB; Stornetta, RL; Guyenet, PG
The Journal of Neuroscience null
Transsynaptic activity-dependent regulation of axon branching and neurotrophin expression in vivo.
Calinescu, AA; Liu, T; Wang, MM; Borjigin, J
The Journal of Neuroscience null
The atypical homeoprotein Pbx1a participates in the axonal pathfinding of mesencephalic dopaminergic neurons.
Sgado, P; Ferretti, E; Grbec, D; Bozzi, Y; Simon, HH
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Wang, Q; Wang, M; Whim, MD
The Journal of Neuroscience null
Cholinergic neurons in the mouse rostral ventrolateral medulla target sensory afferent areas.
Stornetta, RL; Macon, CJ; Nguyen, TM; Coates, MB; Guyenet, PG
Brain Structure &Amp; Function null
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