AB1506
Anti-Glutamate Receptor 2 & 3 Antibody
Chemicon®, from rabbit
Szinonimák:
AMPA-selective glutamate receptor 2, AMPA-selective glutamate receptor 2, glutamate receptor 2, glutamate receptor, ionotropic, AMPA 2
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(4)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
polyclonal
tisztítva
affinity chromatography
faj reaktivitás
hamster, mouse, human, guinea pig, monkey, gerbil, rat
gyártó/kereskedő neve
Chemicon®
technika/technikák
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... GRIA2(2891)
Általános leírás
Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcategorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition.
Egyediség
Expected to cross-react with human, rat, monkey, gerbil, guinea pig, and hamster.
It recognizes both GluR2 and GluR3 which have nearly identical carboxy terminal sequences. Western blot analysis of rat brain shows two poorly separated bands which comigrate with GluR2 and GluR3 expressed in transfected cells. GluR2 migrates slightly ahead of GluR3. No cross-reaction with GluR1 or GluR4. The antibody appears to recognize GluR 2 & 3 in several other species, but has not been extensively characterized in these cases.
Immunogen
Carboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI).
Epitope: C-terminus
Alkalmazás
Immunohistochemistry:
A previous lot was used on paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 µg/mL.
Immunoprecipitation:
When linked to immobilized Protein A, a previous lot of this antibody immunoprecipitated detergent-solubilized receptor from brain or transfected cells.
A previous lot was used on paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 µg/mL.
Immunoprecipitation:
When linked to immobilized Protein A, a previous lot of this antibody immunoprecipitated detergent-solubilized receptor from brain or transfected cells.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
This Anti-Glutamate Receptor 2 & 3 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 2 & 3.
Minőség
Evaluated by Western Blot on rat brain lysate.
Western Blot Analysis:
1:1000 dilution of this antibody detected glutamate receptor 2 & 3 on 10 μg of rat brain lysate.
Western Blot Analysis:
1:1000 dilution of this antibody detected glutamate receptor 2 & 3 on 10 μg of rat brain lysate.
Cél megnevezése
108 kDa
Fizikai forma
ImmunoAffinity Purified
Purified rabbit polyclonal serum lyophilized from buffer containing 0.01M phosphate, 0.09M NaCl, pH 7.2. 1% BSA and 0.1% sodium azide.
Tárolás és stabilitás
Maintain lyophilized material at +2-8°C or -20°C for up to 6 months after date of receipt.
Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks at +2-8°C and reused repeatedly.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks at +2-8°C and reused repeatedly.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analízis megjegyzés
Control
Human brain tissue and extracts from mouse brain and rat brain tissue.
Human brain tissue and extracts from mouse brain and rat brain tissue.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
javasolt
Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Tárolási osztály kódja
13 - Non Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Eunice Y Yuen et al.
The Journal of physiology, 588(Pt 13), 2361-2371 (2010-05-06)
Cofilin, the major actin depolymerizing factor, modulates actin dynamics that contribute to spine morphology, synaptic transmission and plasticity. Much evidence implicates the cofilin inactivation kinase LIMK in synaptic function, but little is known about the cofilin activation phosphatase Slingshot in
ZO-1 and the spatial organization of gap junctions and glutamate receptors in the outer plexiform layer of the mammalian retina.
Puller, C; de Sevilla Muller, LP; Janssen-Bienhold, U; Haverkamp, S
The Journal of Neuroscience null
Andreas T Sørensen et al.
eLife, 5 (2016-09-24)
Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed
Jillian N Moretto et al.
Brain structure & function, 222(5), 2405-2419 (2017-02-13)
Although a great deal of information is available about the circuitry of the mossy cells (MCs) of the dentate gyrus (DG) hilus, their activity in vivo is not clear. The immediate early gene c-fos can be used to gain insight
Jacqueline Flores-Otero et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(51), 14023-14034 (2007-12-21)
A unifying principle of sensory system organization is feature extraction by modality-specific neuronal maps in which arrays of neurons show systematically varied response properties and receptive fields. Only beginning to be understood, however, are the mechanisms by which these graded
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással