The components of this kit are sufficient for 36 assays and include 2-Aminobenzamide, DMSO, Glacial Acetic Acid, and Sodium Cyanoborohydride as a reductant. This kit does not include the clean-up cartridges, product G4548, which are required for use. These are preset quantities. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/610/790/pp0520bul.pdf
PP0520
GlycoProfile™ 2-AB Labeling Kit
Synonym(s):
2-AB Labeling Kit
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Pricing and availability is not currently available.
Quality Segment
General description
The GlycoProfile™ labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosylphosphatidylinositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.
Application
While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.
GlycoProfile™ 2-AB Labeling Kit has been used for the labelling of N-linked glycans from human IgG, Bovine fetuin, and human epididymis protein 4. It has also been used for labelling free oligosaccharides from mouse spleen.
Legal Information
Labeling of glycans with 2-AB is covered under US Patent No. 5,747,347 and its foreign equivalents.
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
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Kit Components Only
Product No.
Description
- 2-AB Labeling Reagent 2 x 5
- Dimethyl sulfoxide (DMSO) 2 x 350
- Acetic acid, glacial 2 x 200
- Reductant (sodium cyanoborohydride) 2 x 6
signalword
Danger
supp_hazards
Storage Class
4.3 - Hazardous materials which set free flammable gases upon contact with water
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A - Water-react. 1
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What is available in the 2-AB labeling kit? Are the weights preset or customer set?
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