P5860
Protease from Bacillus sp.
liquid, ≥8 U/g
Synonym(s):
Esperase® 8.0L
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About This Item
Specific activity:
≥8 U/g
Pricing and availability is not currently available.
Application
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Product P5860 is secreted from Bacillus sp. It is a serine-type protease characterized by excellent perfomance at elevated temperature and pH. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics.[1] The enzyme has been used to test UV radiation as an environmental friendly, non-thermal technology for sterilizing enzymes used in the food industry.
Biochem/physiol Actions
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typcially stabilized by Ca2+ and have high isoelectric points[1].
Legal Information
A product of Novozyme corp.
Esperase is a registered trademark of Novozymes Corp.
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This Item | |||
|---|---|---|---|
| specific activity ≥8 U/g | specific activity ≥16 U/g | specific activity ≥16 U/g | specific activity - |
| form liquid | form liquid | form liquid | form granular |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 20--30 kDa | mol wt 20-30 kDa | mol wt 20--30 kDa | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Elodie Lefebvre et al.
Materials science & engineering. C, Materials for biological applications, 82, 210-216 (2017-10-14)
Chronic wound colonization by bacterial biofilms is common and can cause various complications. An anti-biofilm strategy was developed around the co-entrapment of a commercially available antiseptic, PHMB (polyhexamethylene biguanide 4mgmL
D R Durham et al.
Journal of bacteriology, 169(6), 2762-2768 (1987-06-01)
An alkalophilic Bacillus sp., strain GX6638 (ATCC 53278), was isolated from soil and shown to produce a minimum of three alkaline proteases. The proteases were purified by ion-exchange chromatography and were distinguishable by their isoelectric point, molecular weight, and electrophoretic
Effect of UV light on microbial proteases: From enzyme inactivation to antioxidant mitigation.
Lante A, et al.
Innovative Food Science & Emerging Technologies, 17, 130-134 (2013)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P5860-50ML | 04061834392453 |
| P5860-250ML | 04061838173676 |