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H7016

Hirudin

from leeches, ≥1,500 ATU/mg protein, lyophilized powder

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About This Item

CAS Number:
8001-27-2
UNSPSC Code:
12352202
EC Number:
232-279-1
MDL number:
MFCD00081692
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Product Name

Hirudin from leeches, lyophilized powder, ≥1,500 ATU/mg protein (ATU = antithrombin units)

biological source

animal (Hirudo verbana)

Quality Level

100, 200

form

lyophilized powder

specific activity

≥1,500 ATU/mg protein (ATU = antithrombin units)

mol wt

7 kDa

solubility

pyridine: soluble, water: soluble

storage temp.

2-8°C

General description

Hirudin is a 7 kDa acidic protein containing 65 amino acid residues that has an isoelectric point of 3.5-4.0. It is not glycosylated and lacks tryptophan, arginine and methionine residues. It is chemically stable between pH 2-12 and at temperatures up to 80°C.

Biochem/physiol Actions

The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It binds thrombin with high affinity, covering more than 20% of its surface area and occluding both the active site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.
The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It forms a high-affinity 1:1 complex with thrombin, occluding both the proteolytic site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation, and has been suggested as the clinically preferred anticoagulant (over heparin and citrate) for its specific mode of action and absence of side effects. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.

Analysis Note

Protein determined by Lowry.

Other Notes

One unit (ATU) will neutralize one NIH unit of thrombin at 37 °C, based on direct comparison to an NIH thrombin reference standard.

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This Item
94581H0393M2272
Hirudin from leeches lyophilized powder, ≥1,500 ATU/mg protein (ATU = antithrombin units)

Sigma-Aldrich

H7016

Hirudin from leeches

Hirudin recombinant from yeast lyophilized powder, white, >1500 U/vial

Sigma-Aldrich

94581

Hirudin recombinant from yeast

Hirudin recombinant, expressed in unspecified host, ≥7,000 ATU/mg protein (ATU = antithrombin units)

Sigma-Aldrich

H0393

Hirudin

Melittin from honey bee venom ≥85% (HPLC)

Sigma-Aldrich

M2272

Melittin from honey bee venom

biological source

animal (Hirudo verbana)

biological source

synthetic, yeast (Pichia pastoris)

biological source

-

biological source

animal (Apis mellifera)

Quality Level

100, 200

Quality Level

100

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

pyridine: soluble, water: soluble

solubility

0.1 M Tris, 0.2 M NaCl, pH 8.4: 1 vial/mL, clear, colorless

solubility

pyridine: soluble, water: soluble

solubility

water: 5.00-5.20 mg/mL, clear, colorless to faintly yellow

mol wt

7 kDa

mol wt

Mr ~6963.5

mol wt

~7 kDa

mol wt

~_2.8 kDa

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000463150
0000463150
0000308079
0000279558
0000279558

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Sarah E Sartain et al.
Journal of immunology (Baltimore, Md. : 1950), 196(2), 832-845 (2015-12-18)
Atypical hemolytic uremic syndrome (aHUS) is a thrombotic microangiopathy with severe renal injury secondary to an overactive alternative complement pathway (AP). aHUS episodes are often initiated or recur during inflammation. We investigated gene expression of the surface complement regulatory proteins
Hanif Khan et al.
Chemical biology & drug design, 89(1), 67-73 (2016-08-03)
Hirudin is clinically an important drug used for the treatment of cardiac diseases, but has never been elucidated for antileishmanial potential. This study was designed to determine the therapeutic utility of hirudin against leishmaniasis. Binding affinities of 28 potent proteinase
Mengzhou Xue et al.
Brain : a journal of neurology, 132(Pt 1), 26-36 (2008-09-06)
Proteases such as matrix metalloproteinases (MMPs) and thrombin are implicated in intracerebral haemorrhage (ICH) but their interactions amongst one another and interdependency remain to be defined. The latter is important since proteases acting through different mechanisms to inflict neurotoxicity would
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Global Trade Item Number

SKUGTIN
H7016-100UN04061833630402
H7016-2KU04061831159455
H7016-500UN04061833630426
H7016-10UN04061833620892

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