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G0154

Sigma-Aldrich

Guillard′s (F/2) Marine Water Enrichment Solution

50 ×, liquid, suitable for plant cell culture

Synonym(s):

seawater medium

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About This Item

UNSPSC Code:
10171502
NACRES:
NA.72

sterility

sterile-filtered

form

liquid

concentration

50 ×

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

General description

Guillard′s (F/2) Marine Water Enrichment Solution 50 X is a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater to support the reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing coastal marine algae (diatoms).

Application

Guillard′s (F/2) Marine Water Enrichment Solution has been used:
  • for culturing Chromera velia CCMP2878 to study coral response to Chromera infection
  • to enrich Baltic Sea water to culture unialgal stock of Symbiodinium strains
  • as a supplement in water culture medium to culture Chaetoceros affinis (CA) inoculum

Guillard′s (F/2) Marine Water Enrichment Solution 50 X, is a concentrate of major nutrients, trace metals and vitamins that can be diluted in filtered seawater to support reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing costal marine algae (diatoms).

Formula variant

Without silicate. With the major nutrients, trace metals and vitamins as described by Guillard (1975).

Reconstitution

Use at 20ml/L of seawater.

Phycology Media composition and preparation from powder

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Girish Beedessee et al.
Scientific reports, 9(1), 1204-1204 (2019-02-06)
Symbiodiniaceae dinoflagellates possess smaller nuclear genomes than other dinoflagellates and produce structurally specialized, biologically active, secondary metabolites. Till date, little is known about the evolution of secondary metabolism in dinoflagellates as comparative genomic approaches have been hampered by their large
Gilles Defrel et al.
Frontiers in bioengineering and biotechnology, 9, 734902-734902 (2021-10-19)
Efficient and reliable genome engineering technologies have yet to be developed for diatoms. The delivery of DNA in diatoms results in the random integration of multiple copies, quite often leading to heterogeneous gene activity, as well as host instability. Transgenic
Shao-En Peng et al.
PeerJ, 8, e9745-e9745 (2020-11-17)
The mutualistic symbiosis between anthozoans and intra-gastrodermal dinoflagellates of the family Symbiodiniaceae is the functional basis of all coral reef ecosystems, with the latter providing up to 95% of their fixed photosynthate to their hosts in exchange for nutrients. However
Willem Stock et al.
FEMS microbiology ecology, 95(11) (2019-10-28)
While different microalgae tend to be associated with different bacteria, it remains unclear whether such specific associations are beneficial for the microalgae. We assessed the impact of bacterial isolates, derived from various marine benthic diatoms, on the growth of several
Manuel Serif et al.
Nature communications, 9(1), 3924-3924 (2018-09-27)
Recently developed transgenic techniques to explore and exploit the metabolic potential of microalgae present several drawbacks associated with the delivery of exogenous DNA into the cells and its subsequent integration at random sites within the genome. Here, we report a
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Protocols

Phycology Media in Plant Tissue Culture

Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

Phycology Media in Plant Tissue Culture

Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

Phycology Media in Plant Tissue Culture

Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

Phycology Media in Plant Tissue Culture

Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

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