C5301

Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse

clone M20, ascites fluid

• Synonym(s): Anti-CARD2, Anti-CK-8, Anti-CK8, Anti-CYK8, Anti-K2C8, Anti-K8, Anti-KO
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162254
• Conjugate: unconjugated
• Clone: M20, monoclonal
• Application: IF, IHC (f), WB
• Citations: 24

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: M20, monoclonal
• mol wt: antigen 52 kDa
• contains: 15 mM sodium azide
• species reactivity: feline, human, rabbit, canine, bovine
• technique(s): immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue, western blot: suitable
• isotype: IgG1
• UniProt accession no.: P05787
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... KRT8(3856)

Description

General description

Cytokeratin is a 52.5kD cytoplasmic structural protein that belongs to type II, neutral-to-basic subfamily. It consists of 29 different proteins which is the characteristic of epithelial and trichocytic cells. Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting. Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.Monoclonal Anti-Cytokeratin 8 (mouse IgG1 isotype) is derived from the M20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation purified from the human breast carcinoma cell line MCF7.

Immunogen

cytokeratin from human breast carcinoma cell line MCF7.

Application

Monoclonal anti-cytokeratin peptide 8 antibody has been used in

• immunofluorescence microscopy
• immunohistochemistry
• dot blotting
• protein preparation from bovine epithelial cells
• immunofluorescence[1]
• western blotting

Biochem/physiol Actions

Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting.

Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable