C2037

Cytochrome c from bovine heart

≥95% based on Mol. Wt. 12,327 basis

• CAS Number: 9007-43-6
• UNSPSC Code: 12352202
• EC Number: 232-700-9
• NACRES: NA.61
• MDL number: MFCD00130890
• Form: powder or crystals
• Assay: ≥95% based on Mol. Wt. 12,327 basis
• Biological source: bovine heart
• Mol wt: 12327 Da

Properties

• biological source: bovine heart
• Quality Level: 300
• assay: ≥95% based on Mol. Wt. 12,327 basis
• form: powder or crystals
• mol wt: 12327 Da
• technique(s): cell based assay: suitable
• suitability: suitable for molecular biology
• UniProt accession no.: P62894
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: cow ... CYC1(512500)

Description

General description

Cytochrome C is a heme protein. It is localized between the inner and outer mitochondrial membranes.

Application

Cytochrome c from bovine heart has been used:

• for cytochrome oxidase staining using the mice brain tissue[1]
• as a component of control mixture to evaluate the accuracy of tandem mass spectra in identifying peptides[2]
• to study its effect on the stability, transport, and retention of biochar colloids (BCs) in saturated porous media under various solution pH and ionic strength (IS) conditions[3]
• as a component of staining solution for the detection of cytochrome oxidase activity in the brain tissue sections of mouse[1]
• as a component in the assay to study its superoxide scavenging efficiency
• as a component of serum-free DMEM media to evaluate non-specific cytochrome C reduction in astrocytes and HT-29 cells[4]

Biochem/physiol Actions

Cytochrome C is a key mediator of oxidative phosphorylation. It mediates the electron transport between complex III and complex IV of the respiratory chain.

Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome.

The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen.

The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.

Preparation Note

Prepared using TCA.

Other Notes

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)