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A7595

α-Amylase from Bacillus amyloliquefaciens

greener alternative

liquid, ≥250 units/g

Synonym(s):

BAN 480L, 1,4-α-D-Glucan Glucano-hydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-560-9
NACRES:
NA.54
MDL number:
EC Number:
3.2.1.1.
Specific activity:
≥250 units/g
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form

liquid

Quality Segment

specific activity

≥250 units/g

mol wt

55 kDa

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

storage temp.

2-8°C

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A7595 is from Bacillus amyloliquefaciens and is supplied as a liquid. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis.[1] α-Amylase from Bacillus amyloliquefaciens has been used to hydrolyze sweetpotato amylopectin to identify cluster structure.[2] The enzyme has been used to hydrolyze sago palm starch to reducing sugars, which are then used for ethanol fermentation by Saccharomyces cerevisiae FNCC 3012. The enzyme catalyzes amylolysis of gelatinised waxy maize starch to produce reducing sugars.[3]

Biochem/physiol Actions

α-Amylase is an endoamylase that randomly hydrolyzes the α-(1,4) glucan linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme works best at high temperatures, in the range of 70-90 ° Celcius.
An endoamylase that randomly hydrolyzes α-(1→4)-glycosidic linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme is active at high temperatures (70−90 °C).
The pH range for the α-amylase of B.amyloliquefaciens is 5.5 to 6.5, with a single maximum at 5.9. The activity is rapidly inactivated below pH 5.0.

Other Notes

One unit is the amount of enzyme which dextrinizes 5.26 g of dry starch per hour under standard conditions.

Legal Information

A product of Novozyme Corp.
BAN is a trademark of Novozymes Corp.

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This Item
A4551A9857A2986
specific activity

≥250 units/g

specific activity

500-1,500 units/mg protein

specific activity

≥150 units/mg protein (biuret)

specific activity

-

form

liquid

form

lyophilized powder

form

powder

form

solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

mol wt

55 kDa

mol wt

58-62 kDa

mol wt

-

mol wt

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

greener alternative category

, Enabling

greener alternative category

, Enabling

greener alternative category

Enabling,

greener alternative category

Enabling,


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3



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Global Trade Item Number

SKUGTIN
A7595-50ML04061833385265
A7595-250ML04061833385241

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