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77617

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, Molecular Biology, 25:24:1

Phenol – chloroform – isoamyl alcohol mixture
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About This Item

CAS Number:
136112-00-0
NACRES:
NA.25
PubChem Substance ID:
329767815
UNSPSC Code:
12352401
MDL number:
MFCD00133763
Grade:
Molecular Biology
Technique(s):
ChIP: suitable, DNA extraction: suitable, DNA purification: suitable
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grade

Molecular Biology

Quality Segment

200

product line

BioUltra

form

liquid

technique(s)

ChIP: suitable, DNA extraction: suitable, DNA purification: suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

7.2-8.3(H2O-phase, after extraction with H2O, 1:1)

density

1.28 g/mL at 20 °C

absorption

cut-off at 360 nm

suitability

suitable for molecular biology

application(s)

agriculture

storage temp.

2-8°C

SMILES string

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

InChI key

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

General description

Phenol-chloroform-isoamyl alcohol mixture (PCI-mixture) is a widely utilized mixture in molecular biology and biochemical research, specifically designed for nucleic acid purification, including DNA and RNA. With its essential composition of 25% phenol, 24% chloroform, and 1% isoamyl alcohol, PCI-mixture plays a pivotal role in separating proteins from nucleic acids during the purification process. Phenol denatures proteins, chloroform creates a distinct layer to trap them, and isoamyl alcohol facilitates the clear separation of these layers, enabling the isolation of purified DNA or RNA in the aqueous phase.
The applications of PCI-mixture are diverse, encompassing RNA isolation, where it efficiently extracts RNA from animal and plant tissues for downstream analyses like reverse transcription and gene expression studies. Further, PCI-mixture also proves instrumental in DNA extraction, enabling efficient recovery for various applications such as DNA minisatellite fingerprinting for genetic identification. As a general purification technique, PCI-mixture ensures high-quality preparations of both DNA and RNA for further research.

Application

Phenol − chloroform − isoamyl alcohol mixture has been used in nucleic acid extraction in bile duct tissue, Drosophila, chromatin and RNA immunoprecipitation in Caenorhabditis elegans and mitochondrial DNA.Further, also in the extraction of DNA for the generation of DNA minisatellite fingerprints[1]

Biochem/physiol Actions

When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.

Features and Benefits

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Free from DNase, RNase, NICKase and protease

Physical form

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 25:24:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Phenol – chloroform – isoamyl alcohol mixture

Chloroform – isoamyl alcohol mixture BioUltra, Molecular Biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Sigma-Aldrich

25668

Chloroform – isoamyl alcohol mixture

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

application(s)

agriculture

application(s)

-

application(s)

-

application(s)

-

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

technique(s)

ChIP: suitable, DNA purification: suitable, DNA extraction: suitable

technique(s)

-

technique(s)

-

technique(s)

GC/GC: suitable

form

liquid

form

liquid

form

liquid

form

liquid

impurities

DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% free alkali (as NH3)

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signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

target_organs

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

174.2 °F

flash_point_c

79 °C

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Questions

1–6 of 6 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Hello, Is this product saturated with Tris-Hcl?

    1 answer

    1. Yes, Tris HCl is used to prepare the 100 mM Tris pH 8.0 saturation buffer.

      Helpful?

  3. Is the packaging configuration on the 100 ml presentation glass, amber bottle?

    1 answer

    1. Yes, this product is packaged in an amber glass bottle.

      Helpful?

  4. Dans la description, il est dit que le produit est "composé de 25 % de phénol, 24 % de chloroforme et 1 % d'alcool isoamylique". Les 50% restant, qu'est-ce que c'est ou est-ce une erreur ?

    1 answer

    1. As reported in the 'Physical Form' section, under 'DESCRIPTION', the remainder of this material is 100 mM TRIS, pH 8.0, containing approximately 0.1% 8-hydroxyquinoline.

      Helpful?

  5. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  6. Is there an overlay layer of aqueous TE buffer in the bottles?

    1 answer

    1. The product is saturated with TRIS-buffer. At the time of filling the product consists of a single phase. It is possible that an aqueous phase will be liberated, particularly at lower temperatures. Should phase separation occur, the upper will be the aqueous Tris.

      Helpful?

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