126579

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free

• Synonym(s): Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free
• CAS Number: 9048-46-8
• MDL number: MFCD00130384
• NACRES: NA.25
• UNSPSC Code: 12352202
• eCl@ss: 32160409
• Assay: ≥95% (Albumin, electrophoresis)
• Form: lyophilized powder
• Technique(s): immunofluorescence: suitable
• Impurities: ≤0.2% fatty acids, ≤3.0% Ash, ≤5% moisture

Properties

• description: Merck USA index - 14, 8468
• Quality Segment: 100
• assay: ≥95% (Albumin, electrophoresis)
• form: lyophilized powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): immunofluorescence: suitable
• impurities: ≤0.2% fatty acids, ≤3.0% Ash, ≤5% moisture
• color: pale green
• solubility: aqueous buffer: 20 mg/mL, water: 20 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free is designed for use in cell culture systems and animal perfusion studies. It has a pH of 7.0 (1% solution).

Bovine serum albumin (BSA), a member of the serum albumin family,[1] contains three domains and two sub-domains under each. It is a non-glycosylated globular, α-helical protein that has 17-disulfide bonds.[2][1]

Application

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free has been used in the preparation of advanced glycation end-products (AGEs).[3] It has also been used to dilute lipopolysaccharide (LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of whole blood cells.[4]

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most important constituent of cell culture media. It aids embryonic stem cells (hESC) differentiation.[5] BSA facilitates the transport of drugs, hormones, and fatty acids.[2] It elicits high solubility and is suitable in the cosmetic and pharmaceutical industries.[6] It is applicable as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[7]

Preparation Note

Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.

Material may take up to 24 h to fully dissolve.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable