The microprotein kit TP0200 is not intended for use above 1 mg/ml
TP0200
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification
sufficient for ~50 manual assays
Sinónimos:
Total Protein Assay Kit
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NACRES:
NA.32
UNSPSC Code:
12352202
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wavelength 500-750nm
Quality Level
usage
sufficient for ~50 manual assays
protein detection level measuring range
0-1 mg/mL
parameter
18-26 °C temperature, 200 μL sample volume
storage temp.
2-8°C
General description
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
Application
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins[1]
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)[2]
- in the stored samples of rat liver microsomal fractions
Biochem/physiol Actions
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
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| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| description wavelength 500-750nm | description - | description - | description - |
| usage sufficient for ~50 manual assays | usage sufficient for ~200 assays | usage - | usage - |
| storage temp. 2-8°C | storage temp. - | storage temp. room temp | storage temp. 2-8°C |
| protein detection level measuring range 0-1 mg/mL | protein detection level measuring range - | protein detection level measuring range - | protein detection level measuring range - |
| parameter 18-26 °C temperature | parameter - | parameter - | parameter - |
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS & Precios
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Clase de almacenamiento
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenido relacionado
Instructions
A Kaliszewska et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 95, 89-95 (2016-07-06)
Cereal proteins are of clinical interest because of their cytotoxic and immunogenic features being associated to allergic processes and intestinal disorders. In addition to gliadins, there has been suggested an important role for non-gluten modulating factors (nGMF) on the onset
Inge Everaert et al.
American journal of physiology. Renal physiology, 318(4), F1030-F1040 (2020-03-10)
Manipulation of circulating histidine-containing dipeptides (HCD) has been shown to affect the development of diabetes and early-stage diabetic nephropathy (DN). The aim of the present study was to investigate whether such interventions, which potentially alter levels of circulating HCD, also
Fei-Fan Cai et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 109(8), 1090-1104 (2020-12-06)
The concept of adding inorganic fillers into hydrogels to form hydrogel nanocomposites often provides advantageous properties which can be exploited for successful 3D biofabrication. In this study, a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| TP0200-1KT | 04061837398001 |
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does it works for samples with 11 mg/ml?
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