The concentration of this product varies from lot to lot and may range from 20 - 40 mg/mL. At the minimum concentration of 20 mg/mL, the 10 mg package size would have a volume of 0.5 mL. This information is published in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/r4642#product-documentation
R4642
Ribonucleasa A from bovine pancreas
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
Sinónimos:
ARNasa, ARNasa A, Ribonucleasa pancreática, Ribonucleato 3′-pirimidinooligonucleotidohidrolasa
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Número CAS:
NACRES:
NA.53
UNSPSC Code:
12352204
MDL number:
Biological source:
bovine pancreas
Concentration:
20-40 mg/mL
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Servicio técnico
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Permítanos ayudarlebiological source
bovine pancreas
Quality Level
grade
Molecular Biology
form
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
mol wt
13.7 kDa, ~13,700
concentration
20-40 mg/mL
suitability
suitable for
foreign activity
Endonuclease and exonuclease, none detected, NICKase and DNase, none detected
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
La ARNasa, ribonucleasa A, es una endorribonucleasa que escinde los enlaces fosfodiéster del ARN monocatenario después de nucleótidos de pirimidina. Ataca el extremo 3′ fosfato (por ejemplo, el pG-pG-pC-pA-pG será escindido para dar pG-pG-pCp y A-pG). La mayor actividad la exhibe con ARN monocatenario. La ARNasa A es un polipéptido monocatenario que contiene cuatro puentes bisulfuro. Al contrario que la ARNasa B, no es una glucoproteína. Las ribonucleasas no hidrolizan el ADN porque el ADN carece de los grupos 2′-OH esenciales para la formación de los intermediarios cíclicos. La ARNasa A también puede hidrolizar el ARN de las muestras proteicas. La ARNasa A puede ser inhibida por alquilación de la His12 o la His119 y activada por sales de potasio y de sodio. La RNAasa se inhibe en presencia de iones de los metales pesados. La ARNasa también es inhibida competitivamente por el ADN.
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
Application
- La ribonucleasa A se utiliza para eliminar el ARN de las preparaciones de ADN plasmídico y ADN genómico, y de las muestras de proteínas.
- La ARNasa se utiliza también en el análisis de la secuencia del ARN y en los análisis de protección.
- La RNasa A se ha utilizado como herramienta para el diseño de medicamentos asistido por ordenador.
- La ARNasa A contribuye al análisis de las secuencias de ARN.
- La ARNasa A hidroliza el ARN contenido en las muestras proteicas.
- La ARNasa A apoya la purificación del ADN.
Suitable for:
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
- Plasmid DNA purification
Features and Benefits
Nuestra muy estable ribonucleasa A, ARNasa A, es idónea para la eliminación del ARN, la secuenciación del ARN y la purificación del ADN.
Analysis Note
Proteína determinada mediante E.
Other Notes
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).
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Este artículo | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| biological source bovine pancreas | biological source - | biological source bovine pancreas | biological source bovine pancreas |
| suitability suitable for | suitability - | suitability - | suitability suitable for mRNA or total RNA extracted from cells and tissues |
| form (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) | form lyophilized | form buffered aqueous solution | form lyophilized powder |
| concentration 20-40 mg/mL | concentration - | concentration - | concentration - |
| mol wt 13.7 kDa, ~13,700 | mol wt 13.7 kDa, ~13,700 | mol wt ~13,700 | mol wt ~13,700 |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| R4642-5G | 04061832857855 |
| R4642-50MG | 04061835574773 |
| R4642-10MG | 04061835518760 |
| R4642-250MG | 04061835513987 |
| R4642-1G | 04061835507160 |
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What is the Total Volume of this product?
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What is the actual concentration of 10MG R4642, Ribonuclease A in terms of mg/ml?
1 answer-
The mg/mL concentration of this product is lot dependent and reported in the product Certificate of Analysis. The specification for the concentration is 20 - 40 mg/mL. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/r4642#product-documentationPlease see the link below to review additional information is the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/984/r4642dat.pdfHelpful?
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When using Product R4642, Ribonuclease A from bovine pancreas (RNase A), do I need to boil the RNase A to inactivate residual DNases?
1 answer-
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity.
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Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?
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The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.
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Product R4642, Ribonuclease A from bovine pancreas (RNase A), is a solution. What does the solution contain?
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The product is supplied in a solution containing 50% glycerol and 10 mM Tris-HCl, pH 8.0.
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What is the shelf life of Product R4642, Ribonuclease A from bovine pancreas (RNase A)?
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This product is stable for at least 2 years when stored properly at -20 °C.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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