P7012

Pepsina from porcine gastric mucosa

lyophilized powder, ≥2,500 units/mg protein (E1%/280)

• Sinónimos: Pepsina A, Pepsina from hog stomach
• Número CAS: 9001-75-6
• UNSPSC Code: 12352204
• eCl@ss: 42010127
• EC Number: 232-629-3
• NACRES: NA.54
• MDL number: MFCD00081840
• Número CE: 3.4.23.1 (BRENDA, IUBMB)
• Specific activity: ≥2,500 units/mg protein (E1%/280)

Propiedades

• form: lyophilized powder
• Quality Segment: 300
• specific activity: ≥2,500 units/mg protein (E1%/280)
• mol wt: 35 kDa
• color: off-white to yellow
• solubility: deionized water: soluble 10 mg/mL, 10 mM HCl: soluble 4 mg/mL (Cold)
• UniProt accession no.: P00791
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• Gene Information: pig ... LOC396892(396892)

Descripción

Application

Puede utilizarse hidrólisis con pepsina para producir los fragmentos F(ab′)₂ de los anticuerpos. pepsina en www.sigma-aldrich.com/enzymeexplorer

Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice[1].

The enzyme from Sigma has been used in the digestion of crude wheat gliadin.[2] It has been used along with other enzymes to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays, using paraffin embedded tissue.[3] It has been used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues.[4] The enzyme has also been used in the preparation of F(ab)₂ fragment from IgG.[5]

Biochem/physiol Actions

Escisión preferente: residuos hidrófobos y aromáticos en las posiciones P1 y P1′. Rompe los enlaces Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe y Phe-Tyr en la cadena β de la insulina

Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.

The enzyme does not cleave at valine, alanine, or glycine linkages. Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion. Pepsin is inhibited by several phenylalanine-containing peptides.[6]

Analysis Note

pH óptimo es 2-4. Activa en urea 4 M y HCl de guanidina 3 M. Estable a 60 ºC. La pepsina se desactiva de manera irreversible a pH 8,0 - 8,5.

E1%/280=14.7

Protein determined by E1%/280

Other Notes

One unit will produce a ΔA₂₈₀ of 0.001 per min at pH 2.0 at 37°C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)

View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.

Información de seguridad

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves