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P3752

Phosphatase, Acid from potato

Name: Phosphatase, Acid from potato
Brand: SIGMA

lyophilized powder, ≥0.5 unit/mg solid

Sinónimos:

(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato

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Número CAS:

9001-77-8

eCl@ss:

42010102

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-630-9

MDL number:

MFCD00131847

Número CE:

3.1.3.2 (BRENDA, IUBMB)

Specific activity:

≥0.5 unit/mg solid

Biological source:

potato

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Propiedades

biological source

potato

Quality Segment

200

form

lyophilized powder

specific activity

≥0.5 unit/mg solid

mol wt

69 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

SMILES string

O(CC(O)C)CC#C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

Descripción

Application

Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, product P3752, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages[1].

Biochem/physiol Actions

Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate[1].

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

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Este artículo	P1146	SRE0048	P1435
Sigma-Aldrich P3752 Phosphatase, Acid from potato	Sigma-Aldrich P1146 Phosphatase, Acid from potato	Sigma-Aldrich SRE0048 Phosphatase, Acid from potato	Sigma-Aldrich P1435 Phosphatase, Acid from sweet potato
biological source potato	biological source -	biological source -	biological source -
specific activity ≥0.5 unit/mg solid	specific activity ≥3.0 units/mg solid	specific activity 3.0-10.0 unit/mg solid	specific activity ≥10.0 units/mg protein (modified Warburg-Christian)
form lyophilized powder	form lyophilized powder	form lyophilized powder	form ammonium sulfate suspension
application(s) diagnostic assay manufacturing	application(s) -	application(s) -	application(s) -
mol wt 69 kDa	mol wt 69 kDa	mol wt -	mol wt 110 kDa
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Lot/Batch Number

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