If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
M8250
MES hydrate
≥99.5% (titration)
Sinónimos:
Ácido 2-(N-morfolino)etanosulfónico hydrate, Ácido 4-morfolinoetanosulfónico
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| A ustedes/SKU | Disponibilidad | Precio |
|---|
Acerca de este artículo
biological source
(Chemical/Synthestic)
Quality Segment
assay
≥99.5% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
cell culture | plant: suitable, immunofluorescence: suitable
impurities
≤5 ppm (Total: Ag, As, Bi, Cd, Cu, Hg, Mo, Pb, Sb, Sn)
color
white
useful pH range
5.5-6.7
pKa
6.1
solubility
water: 0.25 g/mL, clear, colorless
suitability
suitable for electrophoresis
application(s)
agriculture
clinical research
diagnostic assay manufacturing
life science and biopharma
storage temp.
room temp
SMILES string
O.OS(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChI key
MIIIXQJBDGSIKL-UHFFFAOYSA-N
General description
Con una excelente hidrosolubilidad e insolubilidad virtual en lípidos, el tampón MES es impermeable a las membranas, lo que garantiza su eficacia en entornos celulares. Sus aplicaciones abarcan el mantenimiento de la estabilidad en medios de cultivo celular, formulaciones amortiguadoras a base de proteínas y tampones de migración electroforética. El tampón MES también puede utilizarse para la purificación de anticuerpos, péptidos, proteínas, componentes sanguíneos y factores de crecimiento. El tampón MES destaca como una mejor alternativa a los agentes potencialmente tóxicos como el cacodilato y los amortiguadores no dipolares como el citrato y el malato. El tampón MES se ha demostrado valioso para ajustar el pH de los medios de crecimiento, estabilizar las disoluciones enzimáticas y como componente del tampón de migración en PAGE en diversos entornos experimentales.
Application
- en los medios MS (Murashige y Skoog) para el crecimiento de plántulas de Arabidopsis.[1]
- en el tampón de citoesqueleto durante los estudios de inmunofluorescencia.
- como tampón de lavado para lavar las microesferas paramagnéticas y resuspenderlas para la unión manosa-BSA
- como componente del medio de crecimiento
Features and Benefits
- Amortiguación eficaz en un pH de 5,5-6,7 (25 °C) con un pKa de 6,1 (25 °C)
- Muy hidrosoluble
- Mínima adsorción a iones metálicos
- Estable en un amplio intervalo de pH
- Baja absorción UV y mínima reactividad
Preparation Note
Other Notes
1 of 1
Este artículo | |||
|---|---|---|---|
| assay ≥99.5% (titration) | assay ≥99.5% | assay ≥99.5% (titration) | assay ≥99.5% (T) |
| technique(s) cell culture | plant: suitable, immunofluorescence: suitable | technique(s) cell culture | mammalian: suitable, immunofluorescence: suitable, nucleic acid detection: suitable | technique(s) - | technique(s) - |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 200 |
| form crystalline powder | form crystalline powder | form crystalline powder | form powder or crystals |
| application(s) agriculture | application(s) advanced drug delivery | application(s) cell analysis | application(s) - |
| storage condition dry at room temperature | storage condition dry at room temperature | storage condition dry at room temperature | storage condition - |
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Clase de almacenamiento
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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