We only use this product for our primary cell and cell line cultures.
D5648
DMEM - high glucose
With ʟ-glutamine, without sodium bicarbonate and sodium pyruvate, powder, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
1 of 2 reviewers received a sample product or took part in a promotion
Sinónimos:
DME, DMEM
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UNSPSC Code:
12352207
NACRES:
NA.75
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Servicio técnico
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Permítanos ayudarleNombre del producto
Medio de Eagle modificado de Dulbecco, glucosa elevada, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
Application
El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».
Dulbecco′s Modified Eagle′s Medium - high glucose has been used as the culture medium for various cell types including the human cervical endocervical epithelial End1/E6E7 cells, human adenocarcinoma epithelial cells (HeLa), human alveolar adenocarcinoma epithelial cells (A549), human osteosarcoma epithelial cells, human diploid cell line , primary hepatocytes and synovial tissue
Preparation Note
Formulated to contain 13.4 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.
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Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form powder | form powder | form powder | form liquid |
| components phenol red: yes | components sodium pyruvate: no | components glucose: high | components sodium pyruvate: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level - | Quality Level 500 | Quality Level 400 |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| D5648-10X1L | 04061835518951 |
| D5648-1L | 04061837468285 |
| D5648-50L | 04061833586549 |
| D5648-10L | 04061833586532 |
| D5648-5L | 04061837468292 |