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D3159

Deoxyribonucleic acid from herring sperm

not suitable for substrate for typical DNase assays (“crude oligonucleotides", <50 bp), degraded

Sinónimos:

Deoxyribonucleic acid, partially degraded from herring sperm, hsDNA

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UNSPSC Code:
41106305
eCl@ss:
32160414
EC Number:
309-566-6
NACRES:
NA.51
MDL number:
MFCD00130921
Biological source:
(Herring sperm)
Form:
powder
Storage temp.:
2-8°C
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biological source

(Herring sperm)

Quality Segment

200

form

powder

suitability

not suitable for substrate for typical DNase assays (“crude oligonucleotides", <50 bp)

storage temp.

2-8°C

General description

Herring Sperm DNA, also known as hsDNA is a natural source of DNA designed for DNA binding agent studies. (1) The detection limits of hsDNA are around 5.0ng and linear ranges of light scattering intensity (I(LS)) is 0.05-2.5 mµg.[1]

Application

Herring sperm DNA (hsDNA) is a natural DNA used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Herring sperm DNA is also used in physicochemical studies of DNA behavior in solution.

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Este artículo
D7290D6898262012
Deoxyribonucleic acid from herring sperm not suitable for substrate for typical DNase assays (“crude oligonucleotides", &lt;50 bp), degraded

Sigma-Aldrich

D3159

Deoxyribonucleic acid from herring sperm

Deoxyribonucleic acid from herring sperm single-stranded for hybridization

Sigma-Aldrich

D7290

Deoxyribonucleic acid from herring sperm

Deoxyribonucleic acid sodium salt from herring testes Type XIV

Sigma-Aldrich

D6898

Deoxyribonucleic acid sodium salt from herring testes

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes Purified DNA from salmon testes. Absorption (0.001% solution in phosphate buffer, pH 7.0, 260 nm): &#8805;0.155.

Millipore

262012

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes

biological source

(Herring sperm)

biological source

(herring sperm)

biological source

-

biological source

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

powder

form

solution

form

solid

form

solid

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

suitability

not suitable for substrate for typical DNase assays (“crude oligonucleotides", <50 bp)

suitability

-

suitability

-

suitability

-

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  

  1. Is it suitable for melting temperature analysis?

    1 answer

    1. This product has not been tested for melting temperature analysis. The product is not considered to be highly purified. This is an application that will have to be validated by the end user.

      It may be helpful to review the literature. See the links below for publications that may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC275468/
      https://pubmed.ncbi.nlm.nih.gov/9056205/
      https://www.researchgate.net/publication/313712083_Probing_the_Effects_on_the_Thermal_Stability_of_Salmon_Sperm_DNA_in_the_Presence_of_Metal_Cations_by_Measuring_DNA_Melting_Temperatures

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. How should I store Product D3159, Deoxyribonucleic acid?

    1 answer

    1. DNA has been stored successfully for several months at 4°C, pH 7.5 - 8, in 10 mM Tris with 1 mM EDTA. The addition of a bacteriostatic agent is not necessary.

      Helpful?

  4. What is the molecular weight range of Product D3159, Deoxyribonucleic acid?

    1 answer

    1. The molecular weight range is 10,000 - 30,000 Daltons. (One base pair is approximately 660 Daltons.)

      Helpful?

  5. How should I reconstitute Product D3159, Deoxyribonucleic acid?

    1 answer

    1. D3159 is soluble in water at 5 mg per mL. Add slowly to stirring water and then heat gently (60° C or less) or dissolve overnight at 4° C

      Helpful?

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