CHOGS

CHOZN^® ZFN-Modified CHO Cell Line

• NACRES: NA.75
• UNSPSC Code: 12352200

Propiedades

• description: CHOZN^® GS^-/- ZFN-modified CHO cell line, glutamine synthetase knockout cell line
• solubility: DMSO: 5 mg/mL, clear
• shipped in: liquid nitrogen
• storage temp.: −196°C

Descripción

General description

CHOZN cell lines were created utilizing Sigma′s proprietary CompoZr Zinc Finger Nuclease (ZFN) technology to create genetic modifications in parental CHO-K1 cells. Cells are banked in chemically defined, animal component free EX-CELL CD CHO Fusion media

ZFNs are a class of engineered DNA-binding proteins and causes a double-strand break. This double-strand break is necessary for site-specific mutagenesis mediated DNA repair. It facilitates targeted genome editing by binding to a user-specified locus. Glutamine synthetase (GS) is one of the most commonly used selectable markers in the biopharmaceutical industry. Glutamine is an essential amino acid for cellular growth. The GS enzyme is responsible for the conversion of glutamate into glutamine. Without this enzymatic activity, cells can no longer synthesize glutamine endogenously and if glutamine is not supplemented in the culture media, the cells will die.

Biochem/physiol Actions

CHOZN cell lines are all subclones of the parental CHO-K1 cell line originated by Puck in 1957.

Packaging

This product is shipped in liquid nitrogen dry vapor shippers.
Liquid nitrogen dry vapor shipper fee (up to a maximum of $125) and freight not included in price of product.
Liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt. A $450 liquid nitrogen dry vapor shipper fee will be charged if not returned within 1 week of delivery.
Learn more about liquid nitrogen dry vapor shipper

Physical form

CHOZN cell lines are provided to customers in vials containing >7.5e6 cells/mL

Legal Information

All CHOZN cell lines are sold under license from Sigma Aldrich. A copy of the research license is provided with purchase of the CHOZN cell lines.

CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable