12-349

Goat Anti-Mouse IgG Antibody, HRP conjugate

Upstate^®, from goat

• Synonym(s): Goat IgG Antibody, HRP-Conjugated Goat Anti-Mouse IgG
• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA, IHC, WB
• Citations: 318

Properties

• biological source: goat
• Quality Level: 100
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
• shipped in: dry ice
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Immunogen

Highly purified whole mouse IgG.

Application

Additional Research Applications
ELISA and Immunohistochemistry:
A working dilution of 1:10,000 to 1:40,000 is suggested for ELISA and 1:500 to 1:2,000 for immunohistochemistry.
Optimal antibody dilution for other applications should be determined by the researcher.

Goat Anti-Mouse IgG Antibody, HRP conjugate is an antibody against Mouse IgG for use in ELISA, IH & WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Biochem/physiol Actions

Recognizes mouse IgG, both heavy and light chains.

Physical form

Purified goat polyclonal IgG in buffer containing conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, and 0.01% gentamicin sulfate.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.

Preparation Note

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Western Blot Analysis:
Suitable for western blotting (dot blot) at 1:1,000 to 1:4,000 dilution.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids