This powder is not sterile. Prepared solutions may be filter-sterilized. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/699/t0665dat.pdf
T0665
Holo-transferrine human
powder, BioReagent, suitable for cell culture, ≥97%
Synonyme(s) :
Sidérophiline, saturée en fer
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352202
EC Number:
234-318-8
NACRES:
NA.75
MDL number:
eCl@ss:
34058011
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Service technique
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Laissez-nous vous aiderbiological source
human plasma
Quality Level
description
Iron-saturated
product line
BioReagent
assay
≥97%
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
HIV, HBsAg and HCV, none detected (tested by FDA approved testing methods), endotoxin, tested
solubility
H2O: 50 mg/mL
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... TF(7018)
Application
L′holo-transferrine humaine a été ajoutée sous forme de supplément au Roswell Park Memorial Institute (RPMI) dans un essai de prolifération de proérythroblastes et dans des milieux de culture pour spectroscopie d′émission à plasma à couplage inductif (ICP-OES).
La transferrine est la principale molécule de fixation et d′administration cellulaire du fer présente dans le sérum. Les systèmes de culture cellulaire sans sérum nécessitent un système d′administration du fer. La transferrine est la forme privilégiée pour l′administration du fer car les cellules traitent le fer lié à la transferrine d′une manière appropriée sur le plan physiologique, grâce aux récepteurs de la transferrine situés à leur surface. L′holo-transferrine humaine est une transferrine de haute affinité qui peut être utilisée avec un large éventail de types de cellules. Elle est chargée en fer avant utilisation et peut être ajoutée directement aux milieux de culture cellulaire à faible teneur en fer ou dépourvus de fer.
Biochem/physiol Actions
Fournit du fer aux cellules sous une forme sans danger et stable sur le plan physiologique.
Preparation Note
Traitée thermiquement à 60 °C (minimum) pendant au moins 10 heures.
Analysis Note
Pureté déterminée par électrophorèse sur gel d′agarose.
Teneur en fer déterminée par ICP et communiquée pour chaque lot.
Disclaimer
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.
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Cet article | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) immunoprecipitation (IP): suitable, tissue culture: suitable |
| form powder | form powder | form powder | form powder |
| biological source human plasma | biological source - | biological source human | biological source human |
| solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in ambient | shipped in ambient | shipped in - | shipped in - |
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Classe de stockage
11 - Combustible Solids
wgk
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Contenu apparenté
Instructions
Jill M Brown et al.
Nature communications, 9(1), 3849-3849 (2018-09-23)
Self-interacting chromatin domains encompass genes and their cis-regulatory elements; however, the three-dimensional form a domain takes, whether this relies on enhancer-promoter interactions, and the processes necessary to mediate the formation and maintenance of such domains, remain unclear. To examine these
Co-operative signalling mechanisms required for erythroid precursor expansion in response to erythropoietin and stem cell factor
Arcasoy O and Jiang X
British Journal of Haematology, 130(1), 121-129 (2005)
Senthil Raja Jayapal et al.
Cell cycle (Georgetown, Tex.), 15(22), 3070-3081 (2016-09-23)
Cyclin A2 is an essential gene for development and in haematopoietic stem cells and therefore its functions in definitive erythropoiesis have not been investigated. We have ablated cyclin A2 in committed erythroid progenitors in vivo using erythropoietin receptor promoter-driven Cre
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| T0665-100MG | 04061837335662 |
| T0665-1G | 04061837335679 |
| T0665-50MG | 04061837335723 |
| T0665-500MG | 04061837335709 |
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Is the powder sterile?
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What is the recommended storage method for stock solutions of item T0665, holo-Transferrin human?
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Stock solutions remain stable for a period of up to two weeks when stored at 4°C.
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What's the process of saturation? Do the cells express directly holo-Tf with iron? Or apo-Tf reacts with iron salts?
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The delivery of iron to cells by transferrins is a receptor-mediated and controlled process. Cells regulate the amount of iron they receive from the extracellular environment by varying transferrin receptor expression. Two iron-loaded transferrin molecules bind to one transferrin receptor. Once bound, they are transferred into acidic endosomes within the cell’s interior. At reduced pH, the iron is released and incorporated into intracellular proteins and the storage molecule ferritin. The apo-transferrin (iron-free) is recycled back to the exterior of the cell and released. Each transferrin receptor cycle can deliver up to four atoms of iron.
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Holo-Tf is described as "iron saturated", but there is a statement "Under normal circumstances, approximately one-third of transferrin iron-binding pockets are filled." I'm confused if the product I got (T0665) is 100% saturated with iron or not.
1 answer-
This product is a Holo-Transferrin form which is generally considered to be iron-saturated. The specification for iron content of this product is 0.10 - 0.35%. Conversely, Apo-Transferrin is considered to be iron deficient. Apo-transferrin product T5391 has an iron content specification of <0.005%. Each Transferrin has the capacity to bind 2 iron (Fe3+) molecules. In a circulating system, the iron content is transitory and rarely (if ever) 100% saturated. This product is tested for total iron content. The number of binding sites that are filled is not measured. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/272/520/t0665pis.pdfHelpful?
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What is the recommended holo-transferrin solvent and how should the dissolved protein be stored?
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This product is tested for solubility in water at 50 mg/mL. Rates are expected to be comparable when dissolving in an aqueous buffer or medium. Solutions are stable for 5-10 days at 2-8°C. This product has not been evaluated for long-term solution stability. However, medium supplements containing transferrin are stable when stored in aliquots at -20°C for up to one year. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/699/t0665dat.pdfHelpful?
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What is the carbohydrate content of human transferrin?
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We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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