The concentration of this product varies from lot to lot and may range from 20 - 40 mg/mL. At the minimum concentration of 20 mg/mL, the 10 mg package size would have a volume of 0.5 mL. This information is published in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/r4642#product-documentation
R4642
Ribonucléase A from bovine pancreas
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
Synonyme(s) :
RNAse a, RNase A, Ribonucléase pancréatique, Ribonucléate 3′-pyrimidino-oligonucléotido-hydrolase
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
| Conditionnement | Référence | Disponibilité | Prix |
|---|
A propos de cet article
Numéro CAS:
NACRES:
NA.53
UNSPSC Code:
12352204
MDL number:
Biological source:
bovine pancreas
Concentration:
20-40 mg/mL
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderbiological source
bovine pancreas
Quality Level
grade
Molecular Biology
form
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
mol wt
13.7 kDa, ~13,700
concentration
20-40 mg/mL
suitability
suitable for
foreign activity
Endonuclease and exonuclease, none detected, NICKase and DNase, none detected
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
La RNase A, ou ribonucléase A, est une endoribonucléase qui clive les liaisons phosphodiester de l′ARN simple brin après les nucléotides pyrimidiques. Elle attaque l′extrémité phosphate en 3′ (par exemple, la séquence pG-pG-pC-pA-pG est clivée en donnant pG-pG-pCp et A-pG). L′activité est maximale avec l′ARN simple brin. La RNase A est un polypeptide à chaîne unique contenant 4 ponts disulfure. Contrairement à la RNase B, ce n′est pas une glycoprotéine. Les ribonucléases n′hydrolysent pas l′ADN, car celui-ci est dépourvu des groupements 2′-OH essentiels à la formation d′intermédiaires cycliques. La RNase A peut également hydrolyser l′ARN présent dans les échantillons protéiques. Elle peut être inhibée par l′alkylation de His12 et His119 et est activée par les sels de potassium et de sodium. Cette RNase est inhibée en présence d′ions de métaux lourds. Elle est également inhibée par l′ADN par un effet de compétition.
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
Application
- La RNase A est utilisée pour supprimer l′ARN présent dans les préparations d′ADN plasmidique et d′ADN génomique ainsi que dans les échantillons protéiques.
- Elle est également employée dans l′analyse de séquences d′ARN et les tests de protection.
- Elle a également servi d′outil dans la conception de médicaments assistée par ordinateur.
- La RNase A supporte l′analyse des séquences d′ARN.
- Elle hydrolyse l′ARN contenu dans les échantillons protéiques.
- La purification de l′ADN est supportée par la RNase A.
Suitable for:
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
- Plasmid DNA purification
Features and Benefits
Notre ribonucléase A, ou RNase A, d′une grande stabilité, convient à l′élimination d′ARN, au séquençage d′ARN et à la purification d'ADN.
Analysis Note
Protéine déterminée par E.
Other Notes
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).
1 of 1
Cet article | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| biological source bovine pancreas | biological source - | biological source bovine pancreas | biological source bovine pancreas |
| suitability suitable for | suitability - | suitability - | suitability suitable for mRNA or total RNA extracted from cells and tissues |
| form (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) | form lyophilized | form buffered aqueous solution | form lyophilized powder |
| concentration 20-40 mg/mL | concentration - | concentration - | concentration - |
| mol wt 13.7 kDa, ~13,700 | mol wt 13.7 kDa, ~13,700 | mol wt ~13,700 | mol wt ~13,700 |
Still not finding the right product?
Explore all of our products under Ribonucléase A from bovine pancreas
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Contenu apparenté
Instructions
Christopher P Selby et al.
The Journal of biological chemistry, 295(50), 17374-17380 (2020-10-23)
In nucleotide excision repair, bulky DNA lesions such as UV-induced cyclobutane pyrimidine dimers are removed from the genome by concerted dual incisions bracketing the lesion, followed by gap filling and ligation. So far, two dual-incision patterns have been discovered: the
Wentao Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(26), 6752-6757 (2017-06-14)
Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon, is the major cause of lung cancer. BaP forms covalent DNA adducts after metabolic activation and induces mutations. We have developed a method for capturing oligonucleotides carrying bulky base adducts, including UV-induced cyclobutane pyrimidine
R Perdigão-Henriques et al.
Oncogene, 35(2), 158-172 (2015-03-24)
The miR-200 family promotes the epithelial state by suppressing the Zeb1/Zeb2 epithelial gene transcriptional repressors. To identify other miR-200-regulated genes, we isolated mRNAs bound to transfected biotinylated miR-200c in mouse breast cancer cells. In all, 520 mRNAs were significantly enriched
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| R4642-1G | 04061835507160 |
| R4642-50MG | 04061835574773 |
| R4642-10MG | 04061835518760 |
| R4642-250MG | 04061835513987 |
-
What is the Total Volume of this product?
1 answer-
Helpful?
-
-
What is the actual concentration of 10MG R4642, Ribonuclease A in terms of mg/ml?
1 answer-
The mg/mL concentration of this product is lot dependent and reported in the product Certificate of Analysis. The specification for the concentration is 20 - 40 mg/mL. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/r4642#product-documentationPlease see the link below to review additional information is the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/984/r4642dat.pdfHelpful?
-
-
When using Product R4642, Ribonuclease A from bovine pancreas (RNase A), do I need to boil the RNase A to inactivate residual DNases?
1 answer-
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity.
Helpful?
-
-
Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?
1 answer-
The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.
Helpful?
-
-
Product R4642, Ribonuclease A from bovine pancreas (RNase A), is a solution. What does the solution contain?
1 answer-
The product is supplied in a solution containing 50% glycerol and 10 mM Tris-HCl, pH 8.0.
Helpful?
-
-
What is the shelf life of Product R4642, Ribonuclease A from bovine pancreas (RNase A)?
1 answer-
This product is stable for at least 2 years when stored properly at -20 °C.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-