The solubility of H9377 has been tested at a concentration of 50 mg/ml in a mixture of 2 parts formic acid to 1 part water, equivalent to a 5% solution. While this may not represent the maximum solubility in the formic acid water mixture, it is the available data. The molecular weight of Hypoxanthine is 136.11 amu, and a 1M solution can be prepared by dissolving 136.11 grams in 1 liter of solvent, provided that there is a suitable solvent for dissolution. This amount can be scaled down to the desired volume. Aliquots should be stored at -20°C for up to 6 months.
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A propos de cet article
Formule empirique (notation de Hill) :
C5H4N4O
Numéro CAS:
Poids moléculaire :
136.11
NACRES:
NA.51
PubChem Substance ID:
UNSPSC Code:
41106305
EC Number:
200-697-3
MDL number:
Beilstein/REAXYS Number:
5811
Assay:
≥99.0%
Biological source:
synthetic (organic)
Form:
powder
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Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.
Hypoxanthine has been used:
- to gavage mice and to evaluate the activity of xanthine oxidase (XO) in pasteurized whole milk in vivo in potassium oxonate (PO) -induced mouse model of hyperuricemia
- as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) medium to cultivate in-vitro cell line (EL-1 cells)[1]
- as a pure standard for the quantification of hypoxanthine in vitreous fluid sample using high-performance liquid chromatography-ultra-violet (HPLC-UV) for the estimation of post-mortem interval
Biochem/physiol Actions
Hypoxanthine acts as an intermediate in purine metabolism. It elicits a vital role as a 5′-base of the anticodon in a few transfer-RNAs. Though similar to guanine (Gua), it is devoid of the exocyclic amino group at C(2) position. It may be used as a substrate to study the specificity and kinetics of hypoxanthine-guanine phosphoribosyl transferases.[2]
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Cet article | |||
|---|---|---|---|
| biological source synthetic (organic) | biological source - | biological source - | biological source - |
| Quality Level 200, 300 | Quality Level 100 | Quality Level 200 | Quality Level - |
| assay ≥99.0% | assay ≥99% (HPLC) | assay ≥99% | assay - |
| form powder | form solid | form powder | form - |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What are the instructions for preparing a 1M hypoxanthine stock solution?
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