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E3019

Esterase from porcine liver

Name: Esterase from porcine liver
Brand: SIGMA

lyophilized powder, ≥15 units/mg solid

Synonyme(s) :

Carboxyl esterase, Carboxylic-ester hydrolase, PLE

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A propos de cet article

Numéro CAS:

9016-18-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-773-7

MDL number:

MFCD00131030

Numéro CE :

3.1.1.1 (BRENDA, IUBMB)

Specific activity:

≥15 units/mg solid

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Propriétés

form

lyophilized powder

Quality Segment

300

specific activity

≥15 units/mg solid

mol wt

168 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Description

Application

Esterase from porcine liver has been used in a study to assess the effect of 5-aminolaevulinic acid peptide prodrugs on photosensitization for photodynamic therapy. Esterase from porcine liver has also been used in a study to investigate how site-specific atherogenic gene expression correlates with subsequent variable lesion development in coronary and peripheral vasculature.

Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

The enzyme from Sigma has been immobilised in hollow fibre ultrafiltration membrane and used for the asymmetric hydrolysis of a meso-diester. Esterase from Sigma has been used to investigate its effect on the release of methacrylic acid (MAA) and 2-hydroxyethyl methacrylate (HEMA) from adhesives formulated under conditions simulating wet bonding.[1] It has been used to examine the ability of carboxylesterase activity to remove permethrin- and bifenthrin-associated toxicity to Ceriodaphnia dubia and Hyalella azteca in a variety of matrices.[2]

Biochem/physiol Actions

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.

Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.[3]

Other Notes

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

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Cet article	E0887	43388	46051
Sigma-Aldrich E3019 Esterase from porcine liver	Sigma-Aldrich E0887 Esterase from rabbit liver	Sigma-Aldrich 43388 Esterase Isoenzyme 1 porcine liver, recombinant	Sigma-Aldrich 46051 Esterase from Bacillus stearothermophilus
specific activity ≥15 units/mg solid	specific activity ≥30 units/mg protein	specific activity ≥30.0 U/g	specific activity ≥0.2 U/mg
application(s) diagnostic assay manufacturing	application(s) -	application(s) -	application(s) -
form lyophilized powder	form lyophilized powder	form crystalline, crystals, powder or flakes	form lyophilized
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
mol wt 168 kDa	mol wt 60 kDa	mol wt -	mol wt -
Quality Level 300	Quality Level 200	Quality Level 100	Quality Level -