The activity of this enzyme is established using a 0.02 M Tris buffer with a starting pH of 8.3 at 0°C. One unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. However, the optimum pH and temperature is highly dependent on the specific application. There are a number of publications regarding the working parameters for this material. Please see the link below to review an example:
https://pubmed.ncbi.nlm.nih.gov/10701452/#:~:text=It%20was%20determined%20that%20the,61706%20Dalton%20by%20gel%20filtration.
C2624
Anhydrase carbonique from bovine erythrocytes
≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder
Synonyme(s) :
Déshydratase carbonique, Hydrolase de carbonate
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-576-6
MDL number:
Specific activity:
≥3,500 W-A units/mg protein
Assay:
≥75% protein basis (biuret), ≥95% (SDS-PAGE)
Biological source:
bovine erythrocytes
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Laissez-nous vous aiderbiological source
bovine erythrocytes
Quality Level
assay
≥75% protein basis (biuret), ≥95% (SDS-PAGE)
form
lyophilized powder
specific activity
≥3,500 W-A units/mg protein
solubility
deionized water: >10 mg/mL
storage temp.
2-8°C
Application
Biochem/physiol Actions
Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications [3].
Other Notes
One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)
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Cet article | |||
|---|---|---|---|
| assay ≥75% protein basis (biuret), ≥95% (SDS-PAGE) | assay - | assay - | assay - |
| biological source bovine erythrocytes | biological source - | biological source bovine erythrocytes | biological source - |
| specific activity ≥3,500 W-A units/mg protein | specific activity ≥2,000 W-A units/mg protein | specific activity ≥3,000 W-A units/mg protein | specific activity - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form powder |
| solubility deionized water: >10 mg/mL | solubility - | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Nicolas Ghéczy et al.
RSC advances, 10(32), 18655-18676 (2020-05-18)
Enzyme-catalysed cascade reactions in flow-through systems with immobilised enzymes currently are of great interest for exploring their potential for biosynthetic and bioanalytical applications. Basic studies in this field often aim at understanding the stability of the immobilised enzymes and their
Abdur Rauf et al.
Chemistry & biodiversity, 14(6) (2017-02-17)
Plant phenolics are known to display many pharmacological activities. In the current study, eight phenolic compounds, e.g., luteolin 5-O-β-glucoside (1), methyl rosmarinate (2), apigenin (3), vicenin 2 (4), lithospermic acid (5), soyasaponin II (6), rubiadin 3-O-β-primeveroside (7), and 4-(β-d-glucopyranosyloxy)benzyl 3,4-dihydroxybenzoate
Joana da Costa Ores et al.
Chemosphere, 88(2), 255-259 (2012-04-20)
This work presents a study of industrially applicable techniques to obtain a biologically supported carbon dioxide capture system, based on the extraction of carbonic anhydrase from bovine blood. Carbonic anhydrase is a metalloenzyme which catalyzes the reversible hydration of carbon
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| C2624-500MG | 04061833484326 |
| C2624-100MG | 04061833484319 |
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What is recommand usage temperature and pH range?
1 answer-
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What is the recommended storage conditions for "Carbonic Anhydrase, from bovine erythrocytes?
1 answer-
The recommended storage conditions for Solution of Carbonic Anhydrase, from bovine erythrocytes may be frozen at –20 °C for future use. It is advised to avoid repeated freezing and thawing. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2–3 uses.
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Is it possible to obtain information about the type of Carbonic Anhydrase (I or II) included in item C2624-carbonic anhydrase?
1 answer-
Product C2624, Carbonic Anhydrase from bovine erythrocytes, with a purity of ≥95% (SDS-PAGE), consists predominantly of CA II, with small levels of CA I and CA IV present.
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What is the isozyme I or isozyme II of Carbonic Anhydrase from bovine erythrocytes (C2624 Carbonic Anhydrase)?
1 answer-
The product C2624, Carbonic Anhydrase from bovine erythrocytes, consists mainly of CA II. However, there are also small levels of CA I and CA IV present.
Helpful?
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