T3573

RANK Ligand/TRANCE human

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonym(s): Osteoclast Differentiation Factor (ODF), Osteoprotegerin Ligand (OPGL), Receptor Activator of NF-KB Ligand (RANKL), TNF-related activation-induced cytokines (TRANCE)
• CAS Number: 207621-35-0
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD01867142
• Form: lyophilized powder
• Assay: >90% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in NSO cells
• Mol wt: glycolysylated recombinant product ~35 kDa by SDS-PAGE, calculated mol wt 23 kDa

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in NSO cells
• assay: >90% (SDS-PAGE)
• form: lyophilized powder
• potency: 1.5-7.5 ng/mL ED₅₀
• mol wt: glycolysylated recombinant product ~35 kDa by SDS-PAGE, calculated mol wt 23 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• UniProt accession no.: O14788
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: human ... TNFSF11(8600)

Description

General description

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) is also known as receptor activator of nuclear factor-κB ligand (RANKL). It is a type II transmembrane signaling receptor. It has a molecular weight of around 35kDa. This protein contains an amino-terminal intracellular tail and a carboxy-terminal extracellular region, that bears a connecting stalk and a receptor-binding domain. Tnfsf11 is located on human chromosome 13q14.11.[1]

Application

RANK Ligand/TRANCE human can be used in in vitro osteoclastogenic assays for osteoclast differentiation.[2]

RANK Ligand/TRANCE human has been used to stimulate differentiation.

Biochem/physiol Actions

Member of the TNF superfamily; induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis; cell surface receptor for RANK Ligand.

RANK Ligand (receptor activator of NF-kB ligand (RANKL) also referred to as TNF-related activation induced cytokines (TRANCE) or osteoprotegerin ligand is a protein that belongs to tumor necrosis factor (TNF) family. It stimulates the mature dendritic cells and induces the cytokine production.[3] RANKL also facilitates bone remodelling and possess an angiogenic activity.[4]

RANK Ligand/TRANCE, a member of the TNF superfamily, induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis. Mouse and human RANK Ligand share 85% amino acid identity. RANK is the cell surface receptor for RANK Ligand.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS and 500 mM NaCl, pH 6.5, with 50 μg BSA per 1 μg as a carrier protein.

Analysis Note

The biological activity is measured by its ability to induce osteoclast differentiation on mouse splenocytes.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)