C8554

Chorionic gonadotropin human

lyophilized powder, suitable for mouse embryo cell culture

• Synonym(s): Choriogonin, HCG
• CAS Number: 9002-61-3
• EC Number: 232-660-2
• MDL number: MFCD00130797
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human urine
• Quality Level: 200
• sterility: aseptically processed
• form: lyophilized powder
• potency: ≥5,000 I.U. per mg
• mol wt: 37.9 kDa; apparent mol wt 37.9 kDa by SDS-PAGE
• drug control: Home Office Schedule 4.2
• technique(s): cell culture | embryo: suitable
• impurities: HBV,HCV and HIV, tested negative
• solubility: H₂O: 1 mL/vial; saline: 1 mL/vial
• UniProt accession no.: P01215
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CGA(1081)

Description

General description

Glycoprotein hormone consisting of a 92-amino acid α-chain which is identical to that of LH, FSH, and TSH; and a distinct 149-amino acid β-chain. Produced by chorionic tissue and responsible for maintaining the corpus luteum during early pregnancy. Also produced by trophoblast cell neoplasms, which are marked by elevated free hCG β-chains and hyperglycosylated hCG.

Note: Actual molecular weight will vary from the theoretical depending upon the degree of glycosylation. Theoretical MW is 37.9 kDa based on native form which contains 2 subunits: α = 14.9 kDa (~10.2 kDa for the polypeptide and ~4.7 kDa for the carbohydrate), and β = 23.0 kDa (~16.0 kDa for the polypeptide and ~7.0 kDa for the carbohydrate). See J. Biol. Chem. 248, 6796-6827 (1973).

Application

Recommended for superovulation induction in mice.

Biochem/physiol Actions

Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals. When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.

Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H361
• Precautionary Statements: P281
• Hazard Classifications: Repr. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable