T5944

Tumor Necrosis Factor-α from rat

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonym(s): Cachectin, TNF-α
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00282383
• Biological source: rat
• Recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE and HPLC)
• Form: powder
• Mol wt: predicted mol wt ~17 kDa
• Impurities: <1 EU/μgtested (LAL test)

Properties

• Product Name: Tumor Necrosis Factor-α from rat, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
• biological source: rat
• Quality Level: 200
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE and HPLC)
• form: powder
• quality: endotoxin tested
• mol wt: predicted mol wt ~17 kDa
• packaging: pkg of 10 μg, pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μgtested (LAL test)
• color: white
• solubility: water: soluble
• UniProt accession no.: P16599
• storage temp.: −20°C

Description

General description

The Tnf (tumor necrosis factor) gene is mapped to rat chromosome 20p12.

Application

TNF-α influences the growth and function of both normal and neoplastic cells. TNF-α causes cytolysis or cytostasis of certain transformed cells being synergistic with γ-interferon in its cytotoxicity.

Tumor Necrosis Factor-α from rat has been used:

• To stimulate inducible nitric oxide synthase (iNOS) expression in the macrophages as an indication of M1 macrophage activation
• To study its effect on pancreatic β cell apoptosis[1]
• To evaluate the effect of progesterone on the expression of tumor necrosis factor (TNF)-α in synovial membrane
• As a blocking antigen in the control, for immunohistochemical analysis

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α), also known as cachectin, plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication, and hematopoiesis. TNF-α is expressed by a variety of cells, with numerous inductive and suppressive agents. It is primarily produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens, and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2.

Tumor necrosis factor-α, also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in anti-tumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is cytotoxic for many transformed cells, but in normal diploid cells, it stimulates proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and induces production of several other cytokines.

Physical form

Lyophilized from an 0.2 μm-filtered buffered solution with bovine serum albumin as a carrier.

Analysis Note

The biological activity is measured in a cytotoxic assay using a TNF-susceptible mouse L929 cell line in the presence of actinomycin D.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable