SYBR Green qPCR is utilized to determine the relative silencing effect of specific shRNAs. The term "validated" refers to shRNA sequences that have been tested and confirmed effective in silencing. This validation involves testing in specific cell lines and measuring the mean knockdown level through lentiviral transduction. The evaluation of knockdown is conducted by measuring mRNA transcript levels post-lentiviral transduction with SYBR Green qPCR and comparing these levels to those achieved with the MISSION pLKO.1-puro Control Transduction Particles (SHC001V). For instance, a mean knockdown level of 0.90 indicates a 90% reduction at the mRNA level. Typically, validation does not extend to the protein level, as the primary target of shRNA is mRNA expression, which subsequently influences protein expression.
SHCLND
MISSION® shRNA
Purified DNA
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About This Item
UNSPSC Code:
41106609
To order shRNA please search for your gene of interest.
General description
Our comprehensive shRNA product offering consists of over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes. The library is available in: gene family sets: gene collections related to specific functional classes such as kinases and ion channels; gene taget sets, sets of shRNA clones that target your favorite gene; and individual shRNA clones. Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state-of-the-art robotics, liquid handling and laboratory information management systems (LIMS) to provide superior quality and service for your RNAi research needs.
MISSION® TRC shRNA purified plasmid DNA format takes the hassle out of preparing DNA from each construct. DNA may be used directly for transient or stable transfection. Additionally these plasmids can be combined with packaging plasmids in a packaging cell line to produce lentiviral particles. 1 μg of DNA is provided for each construct in the target set.
Application
MISSION® shRNA has been used:
- in the generation of ZO-1 (zonula occludens-1) knockdown
- to knock down LIF (leukemia inhibitory factor) expression in isogenic MCF10 model
- to knock down nuclear factor erythroid 2 like 1, in n MIN6 pancreatic β-cells
Other Notes
To see protocols and application data please visit sigma.com/shrna.
Legal Information
Use of this product is subject to one or more license agreements.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
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| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SHCLND | 04061835574056 |
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How is the knockdown measured for validated shRNA clones?
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